A potential mechanism for regulating myosin I binding to membranes in vivo.
A potential mechanism for regulating myosin I binding to membranes in vivo.
复制标题
调节体内肌球蛋白 I 与膜结合的潜在机制。
DOI：
10.1016/s0014-5793(00)02137-2
复制
发表时间：
2000
期刊：
影响因子：
3.5
通讯作者：
Titus,MA
中科院分区：
文献类型：
--
作者：
Senda,S;Titus,MA
Myosin Is are associated with specific membranes, however, the mechanism for regulating their intracellular localization is unclear. As a first step towards understanding this mechanism, membrane rebinding assays using Dictyostelium myoB were performed. Crude, cytosolic myoB bound to intact, but not to NaOH-treated plasma membranes. In contrast, partially purified myoB binds to both intact and NaOH-treated plasma membranes. Chemical cross-linking of cytosolic myoB yielded several products, whereas none were found with the partially purified myoB. These results suggest a model where proteins regulating the specific binding of myoB to the plasma membrane may exist both in the cytosol and on the plasma membrane.
