Calcium regulation of pigment transport in vitro.
Calcium regulation of pigment transport in vitro.
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DOI：
10.1083/jcb.106.1.111
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发表时间：
1988-01
期刊：
影响因子：
--
通讯作者：
Ward JB
中科院分区：
文献类型：
--
作者：
McNiven MA;Ward JB
钙参与许多细胞运动事件的调节。在这项研究中，我们已经研究了不同的Ca 2+浓度的作用，在体外运输的色素内培养的色素细胞。用Brij去污剂处理1-2分钟的细胞被剥离其质膜，使其细胞骨架和相关的色素颗粒暴露于外部环境。我们发现，当加入1 mM MgATP 2-和10（-7）M游离Ca 2+时，可诱导逆行色素转运（聚集），而当保持1 mM MgATP 2-时，游离Ca 2+浓度降低至10（-8）M，可导致色素的顺行转运（再分散）。这些Ca 2+调节的运动是ATP依赖性的，但显然是独立的cAMP和不敏感的钙调素抑制剂。这里报道的意见提供了新的证据表明，游离Ca 2+的浓度的行为，以调节细胞内细胞器运输的方向。
Calcium has been implicated in the regulation of many cellular motility events. In this study we have examined the role of different Ca2+ concentrations on the in vitro transport of pigment within cultured chromatophores. Cells treated with Brij detergent for 1-2 min were stripped of their plasma membranes, leaving their cytoskeleton and associated pigment granules exposed to the external milieu. We found that retrograde pigment transport (aggregation) is induced upon addition of 1 mM MgATP2- with 10(-7) M free Ca2+, while an orthograde transport (redispersal) of pigment results from lowering the concentration of free Ca2+ to 10(-8) M while maintaining 1 mM MgATP2-. These Ca2+-regulated movements are ATP dependent but are apparently independent of cAMP and insensitive to calmodulin inhibitors. The observations reported here provide novel evidence that the concentration of free Ca2+ acts to regulate the direction of intracellular organelle transport.