1H NMR and NOE studies of the purple acid phosphatases from porcine uterus and bovine spleen.
1H NMR and NOE studies of the purple acid phosphatases from porcine uterus and bovine spleen.
复制标题
猪子宫和牛脾脏紫色酸性磷酸酶的 1H NMR 和 NOE 研究。
DOI：
10.1021/bi00138a004
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Averill,BA
中科院分区：
文献类型：
--
作者：
Wang,Z;Ming,LJ;QueJr,L;Vincent,JB;Crowder,MW;Averill,BA
化学系，弗吉尼亚大学，夏洛茨维尔，弗吉尼亚州22901接收1992年1月23日;修订的Mandarin pt接收1992年4月2日摘要：从猪子宫（也称为子宫铁蛋白，Uf）和牛脾（BSPAP）和它们与钨酸盐的复合物的二铁活性位点的NMR和NOE技术进行了比较。还原BSPAP的NMR谱的顺磁位移特征与还原Uf的相似，而钨酸盐配合物的谱几乎相同。这些观察结果表明，两个活性位点非常相似，与两种酶中发现的> 90%序列同源性一致。His NH共振的核Overhauser效应（NOE）实验表明，两种酶中Fe（III）-His残基均为Ne配位，而Fe（II）-His残基均为Hj配位。基于上述NMR和NOE结果，我们先前提出的Uf的双核铁活性位点的模型[Scarrow，R.，Pyrz，J.W.，& Que，L.，Jr.等人（1990）J. Am. Soc.112，657-665]被证实，精炼，并发现代表BSPAP的二铁中心。
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901 Received January 23, 1992; Revised Manuscript Received April 2, 1992 abstract: The diiron active sites of the purple acid phosphatases from porcine uterus (also called uteroferrin, Uf) and bovine spleen (BSPAP) and their complexes with tungstate are compared by NMR and NOE techniques. The paramagnetically shifted features of the NMR spectrum of reduced BSPAP are similar to those of reduced Uf, while the spectra of the tungstate complexes are almost identical. These observations suggest that the two active sites are quite similar, in agreement with the> 90% sequence homology found in the two enzymes. Nuclear Overhauser effect (NOE) experiments on the His NH resonances show that the Fe (III)-His residue is Ne-coordinated, while the Fe (II)-His is Hj-coordinated in both enzymes. On the basis of the above NMR and NOE results, our previously proposed model for the dinuclear iron active site of Uf [Scarrow, R.€., Pyrz, J. W., & Que, L., Jr.(1990) J. Am. Chem. Soc. 112, 657-665] is corroborated, refined, and found to represent the diiron center of BSPAP as well.