Cytotoxic activities of normal cultured human T cells.
Cytotoxic activities of normal cultured human T cells.
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正常培养的人类 T 细胞的细胞毒活性。
DOI：
10.4049/jimmunol.121.4.1270
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发表时间：
1978
影响因子：
4.4
通讯作者：
R. Herberman
中科院分区：
文献类型：
--
作者：
J. Álvarez;M. de Landázuri;G. Bonnard;R. Herberman
研究了在含有PHA刺激的淋巴细胞的条件培养基（CM）的培养基中连续培养的正常人T细胞表现出三种已知形式的细胞介导的细胞毒性的能力：凝集素诱导的细胞毒性（LICC），自然杀伤细胞（NK）活性和抗体依赖性细胞毒性（ADCC）。培养的T细胞（CTC）是非常有效的LICC介质，即使在非常低的攻击者-靶细胞比率下在不同凝集素的存在下也具有细胞毒性，并且针对不同类型的靶标。当在不添加凝集素的情况下进行测试时，CTC表现出低程度的自发细胞毒性。然而，这种自发的细胞毒性可能不是由于常规NK细胞，因为CTC未能显示出显著数量的具有IgG的Fc受体（FcR）的细胞，并且没有可检测的ADCC活性。CTC可以代表富集多克隆活化T细胞的群体，其具有针对多种同种异体靶细胞的低自发细胞毒性，这通过在51 Cr释放测定期间添加凝集素而大大增强。
Normal human T cells grown in continued cultures in medium containing conditioned medium (CM) from PHA-stimulated lymphocytes were studied for their ability to manifest three known forms of cell-mediated cytotoxicity: lectin-induced cellular cytotoxicity (LICC), natural killer cell (NK) activity, and antibody-dependent cellular cytotoxicity (ADCC). The cultured T cells (CTC) were very effective mediators of LICC, being cytotoxic even at very low attacker-target cell ratios in the presence of different lectins, and against different types of targets. When tested without the addition of lectin, the CTC demonstrated a low degree of spontaneous cytotoxicity. This spontaneous cytotoxicity might not be due to conventional NK cells however, since the CTC failed to show significant numbers of cells with Fc receptors (FcR) for IgG, and had no detectable ADCC activity. CTC could represent a population enriched in polyclonal activated T cells with low spontaneous cytotoxicity against a variety of allogeneic target cells, which is greatly enhanced by the addition of lectins dur ing the 51Cr release assay.