Enhancement of Candida parapsilosis catalyzing deracemization of (R,S)-1-phenyl-1,2-ethanediol to its (S)-enantiomer by a highly productive "two-in-one" resin-based in situ product removal strategy
Enhancement of Candida parapsilosis catalyzing deracemization of (R,S)-1-phenyl-1,2-ethanediol to its (S)-enantiomer by a highly productive "two-in-one" resin-based in situ product removal strategy
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DOI：
10.1016/j.biortech.2010.05.099
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发表时间：
2010-11-01
影响因子：
11.4
通讯作者：
Nie, Yao
中科院分区：
文献类型：
--
作者：
Hu, Qingsen;Xu, Yan;Nie, Yao
在这项研究中，一个高度有效的过程中，近平滑假丝酵母催化的消旋1-苯基-1，2-乙烷（PED）的去外消旋，基于树脂为基础的原位底物进料和产品去除（ISSFPR）的方法。选择树脂H103并用于将（R）-PED和（S）-PED在水相中的组合浓度保持在约15 g/l。在143 g/l H103树脂存在下，50 g/l外消旋底物转化为（S）-PED，对映体过量（ee）为99.3%，产率为92%，而初始反应仅在底物浓度为15 g/l或更低时才能获得ee > 99%。据我们所知，这是第一份关于应用基于树脂的ISSFPR技术提高微生物立体转化效率的报告。(C)2010爱思唯尔有限公司保留所有权利。
In this study, a highly efficient process for Candida parapsilosis-catalyzed deracemization of racemic 1-phenyl-1,2-ethanediol (PED) was described, based on a resin-based in situ substrate feeding and product removal (ISSFPR) methodology. The resin H103 was selected and used to keep the combined concentration of (R)-PED and (S)-PED at about 15 g/l in aqueous phase. In the presence of 143 g/l H103 resin, 50 g/l of racemic substrate was converted to (S)-PED with 99.3% enantiomeric excess (ee) in 92% yield, while the initial reaction could afford an ee > 99% only when the substrate concentration was 15 g/l or lower. To our knowledge, this was the first report on the application of a resin-based ISSFPR technique for enhancing the efficiency of microbial stereoinversion. (C) 2010 Elsevier Ltd. All rights reserved.