Mating-type gene switching in Saccharomyces cerevisiae.
Mating-type gene switching in Saccharomyces cerevisiae.
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DOI：
10.1016/0168-9525(92)90329-3
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发表时间：
1992-12
期刊：
影响因子：
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通讯作者：
J. Haber
中科院分区：
文献类型：
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作者：
J. Haber
(参考文献14，15)人们对SIR蛋白的功能知之甚少；例如，没有一个SIR蛋白似乎直接与沉默的复制DNA 16结合。Sir2似乎影响染色体结构的其他方面，因为SIR2缺失突变体显示rDNA重组17水平升高。SIR基因产物之间的相互作用是从研究表明不同SIR基因TM的多个副本抑制SIR突变而推断出来的。蛋白质转乙酰酶和组蛋白H4的参与可能表明沉默涉及组蛋白乙酰化的变化；然而，氨基末端的赖氨酸被精氨酸取代允许沉默，尽管丙氨酸取代启动沉默基因14，15。
(Refs 14, 15). Very little is known about the functions of the SIR proteins; for example, none of them appears to bind directly to the silent copy DNA 16. SIR2 appears to affect other aspects of chromosome structure, as a sir2 deletion mutant shows elevated levels of rDNA recombination 17. Interactions between SIR gene products have been inferred from studies showing suppression of sir mutations by multiple copies of a different SIR gene TM. The involvement of protein transacetylases and of histone H4 might suggest that silencing involves changes in histone acetylation; however, replacements of lysines in the amino terminus by arginines allows silencing, though alanine replacements turn on the silent gene 14, 15.