Primary responses of human T cells to mycobacteria: a frequent set of γ/δ T cells are stimulated by protease‐resistant ligands
Primary responses of human T cells to mycobacteria: a frequent set of γ/δ T cells are stimulated by protease‐resistant ligands
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人类 T 细胞对分枝杆菌的主要反应：一组频繁的 γ/γ T 细胞受到蛋白酶抗性配体的刺激
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
H. Wagner
中科院分区：
文献类型：
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作者：
K. Pfeffer;B. Schoel;H. Gulle;S. Kaufmann;H. Wagner
从人外周血 T 细胞中选择表达 T 细胞受体 α/β 或 γ/δ 的 T 淋巴细胞亚群，并测定对分子质量分级的分枝杆菌裂解物的增殖反应。 α/β T 细胞主要对 > 30 kDa 的组分发生反应，而 γ/δ T 细胞优先对 < 3 kDa 的组分发生反应。蛋白酶消化消除了对 α/β T 细胞的刺激活性，证实了 α/β T 细胞对蛋白质成分有反应。相比之下，γ/δ T 细胞识别的成分对蛋白酶消化具有抵抗力。在有限稀释研究中，刺激裂解物的蛋白酶处理实际上未改变 γ/β T 细胞的增殖频率，而 α/β T 细胞的增殖频率几乎检测不到。此外，只有少数 γ/δ T 细胞对 65-kDa 热休克蛋白有反应。我们的数据表明，与 α/β T 细胞不同，γ/δ T 细胞对对强力蛋白酶消化具有抵抗力的分枝杆菌成分有反应。
T lymphocyte subsets expressing either T cell receptor α/β or γ/δ were selected from human peripheral blood T cells and proliferative responsesto molecular mass‐fractionated mycobacterial lysates were determined. α/β T cells primarily responded to fractions > 30 kDa whereas γ/δ Tcells preferentially reacted to fractions < 3 kDa. Protease digestion abolished the stimulating activities for α/β T cells, confirming that α/β Tcells respond to protein components. In contrast, components recognized by γ/δ T cells proved resistant to protease digestion. In limiting dilution studies, frequencies of proliferating γ/β T cells remained virtually unaltered by protease treatment of stimulating lysates, while those of α/β T cells became almost undetectable. Furthermore, only few γ/δ T cells responded to the 65‐kDa heat‐shock protein. Our data indicate that, unlike α/β T cells, γ/δ T cells respond to mycobacterial components which are resistant to vigorous protease digestion.
γ/δ T 细胞受体的结构、功能和分子遗传学。
影响因子：
29.7
作者：
Raulet,DH
通讯作者：
Raulet,DH