Pairing and synapsis in wild type Arabidopsis thaliana
Pairing and synapsis in wild type Arabidopsis thaliana
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DOI：
10.1007/s10577-008-1220-z
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发表时间：
2008-08-01
影响因子：
2.6
通讯作者：
Cunado, Nieves
中科院分区：
文献类型：
--
作者：
Lopez, Eva;Pradillo, Monica;Cunado, Nieves
利用扩散技术对野生型拟南芥花粉母细胞（PMCs）的前期I核进行了结构分析。在细线期，所有的染色体发育完全轴向元件之前，突触前对齐观察。配对和联会开始在区域接近端粒在偶线早期。间质联会复合体（SC）伸展被发现发生在几个网站每二价在偶线中期。在单个二价体中，广泛的SC形成区域通常与其他未突触的广泛区域同时存在。在同一个核中，一个二价体可能有几个SC片段，而其他二价体只有几个。传统的香气不像其他植物那样明显。五个粗线期二价体的长度测量允许粗线期核型的阐述。粗线期染色质压缩在拟南芥中显着低于在其他物种中观察到的分析，这是一个较高的重组率（厘摩每兆碱基）。
A spreading technique was used to perform a structural analysis of prophase I nuclei in pollen mother cells (PMCs) of wild-type Arabidopsis thaliana. In leptotene, all chromosomes developed fully axial elements before a presynaptic alignment was observed. Pairing and synapsis start in regions close to the telomeres at early zygotene. Interstitial synaptonemal complex (SC) stretches were found to occur at several sites per bivalent at mid zygotene. Within individual bivalents, extensive regions of SC formation often existed at the same time as other extensive regions that were unsynapsed. Also in the same nucleus, one bivalent might have several SC segments, while other bivalents have only a few. The classical bouquet was not so evident as in other plant species. Length measurements of the five pachytene bivalents have allowed the elaboration of a pachytene karyotype. Pachytene chromatin compaction in Arabidopsis was significantly less than that observed in the other species analysed and this is paralleled with a higher recombination rate (centimorgans per megabase).