Surface characteristics and proteomic analysis insights on the response of Oenococcus oeni SD-2a to freeze-drying stress
Surface characteristics and proteomic analysis insights on the response of Oenococcus oeni SD-2a to freeze-drying stress
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酒酒球菌 SD-2a 对冻干应激反应的表面特征和蛋白质组学分析见解
DOI：
10.1016/j.foodchem.2018.04.137
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发表时间：
2018
期刊：
影响因子：
8.8
通讯作者：
Zhang Guoqiang
中科院分区：
文献类型：
--
作者：
Yang Kun;Liu Miaomiao;Wang Jing;Hassan Hamada;Zhang Jie;Qi Yiman;Wei Xinyuan;Fan Mingtao;Zhang Guoqiang
酒酒球菌Oenococcus oeni（O. oeni）诱导苹果酸-乳酸发酵以改善葡萄酒质量。然而，其冻干抗性机制的分子基础仍不清楚。在这项工作中，O。对oeniSD-2a无冻干（No-FD）、有冻干（FD）和有谷氨酸盐（MSG）的冻干（FD-MSG）进行了研究。流式细胞仪结果显示FD细胞损伤严重，FD-MSG细胞膜传递性降低。同时，扫描电镜观察FD和FD-MSG细胞的胞外多聚物（EPS）。生物信息学分析表明，不同的蛋白质参与碳，脂肪和核酸代谢，应激反应，氧化还原酶活性和信号传感。在鉴定的蛋白质中，突出显示的蛋白质是那些参与多糖产生和信号传感的冻干抗性和环丙烷脂肪酸代谢的味精添加。
Oenococcus oeni(O. oeni) induces malolactic fermentation to improve wine quality. However, the molecular basis of mechanisms involved in its freeze-drying resistance remains unclear. In this work,O. oeniSD-2a without freeze-drying (No-FD), with freeze-drying (FD) and freeze-drying with monosodium glutamate (MSG) (FD-MSG) were investigated. Flow cytometry results showed severe cell damage in FD cells and less membrane transmissibility in FD-MSG cells. Meanwhile, extracellular polymeric substances (EPS) were detected in FD and FD-MSG cells by scanning electron microscopy. Bioinformatic analysis revealed that varying proteins were involved in carbon, lipid and nucleic acids metabolism, stress response, oxidoreductase activity and signal sensing. Among the identified proteins, the highlighted proteins were those involved in polysaccharides production and signal sensing for freeze-drying resistance and cyclopropane fatty acid metabolism for MSG addition.