High-Efficiency EpCAM/EGFR-PLGA Immunomagnetic Beads with Specific Recognition on Circulating Tumor Cells in Colorectal Cancer
High-Efficiency EpCAM/EGFR-PLGA Immunomagnetic Beads with Specific Recognition on Circulating Tumor Cells in Colorectal Cancer
复制标题
对结直肠癌循环肿瘤细胞具有特异性识别的高效 EpCAM/EGFR-PLGA 免疫磁珠
DOI：
10.1166/jbn.2017.2394
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发表时间：
2017-07-01
影响因子：
2.9
通讯作者：
Li, Peiyong
中科院分区：
文献类型：
--
作者：
Cao, Yan;Liang, Xiaofei;Li, Peiyong
探讨上皮细胞粘附分子（EpCAM）和表皮生长因子受体（EGFR）PLGA磁珠在大肠癌循环肿瘤细胞（CTC）分离鉴定中的分离效率。以羧基聚乳酸-羟基乙酸（PLGA-COOH）和壳聚糖十六烷基三甲基铵（HQCMC）为载体，包裹Fe 3 O 4磁性纳米粒子，连接EpCAM抗体和EGFR抗体，制备免疫磁珠（IMMS）。对35例大肠癌患者的新鲜血液进行检测。采用IMMS分离技术对EpCAM和EGFR免疫磁珠捕获的CTCs进行计数，并与患者病情和临床病理资料进行比较。结果表明，自制的荧光探针和GPM能有效地特异性识别和捕获不同类型的结直肠癌CTC，与临床结果相吻合。此外，GPM捕获的CTC与EGFR基因突变的检测结果一致。
To investigate the separation efficiency of epithelial cell adhesion molecule (EpCAM) and epidermal growth factor receptor (EGFR) PLGA magnetic beads in colorectal cancer circulating tumor cells (CTCs) separation and identification. The carboxylic polylactic acid-glycolic acid (PLGA-COOH) and chitosan hexadecyltrimethylammonium (HQCMC) were used to encapsulate Fe3O4 magnetic nanoparticle, connect EpCAM antibody and EGFR antibody to prepare immunomagnetic beads (IMMS). The fresh blood of 35 cases of colorectal cancer patients was collected and detected. The CTCs captured by EpCAM immunomagnetic beads (EPM) and EGFR immunomagnetic beads (GPM) were counted by IMMS separation technology and compared with the patient's condition and the clinical pathological data. The results showed that the homemade EPM and GPM could effectively specific recognition and capture different colorectal cancer CTCs, which was matching with the clinical results. In addition, GPM captured CTCs were consistent with the detection results of EGFR gene mutation.