Effect of hyperbaric oxygen on MMP9/2 expression and motor function in rats with spinal cord injury.
Effect of hyperbaric oxygen on MMP9/2 expression and motor function in rats with spinal cord injury.
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发表时间：
2015-09
影响因子：
0.1
通讯作者：
Ying-Nuo Hou;W. Ding;Yong Shen;Dalong Yang;Lin‐feng Wang;P. Zhang
中科院分区：
文献类型：
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作者：
Ying-Nuo Hou;W. Ding;Yong Shen;Dalong Yang;Lin‐feng Wang;P. Zhang
研究高压氧干预对脊髓损伤建模后神经再生微环境的影响，探讨脊髓损伤大鼠神经再生和功能恢复的可能机制。98只成年雌性SD大鼠，取成功模型90只，按随机块法分为假手术组、脊髓损伤组和高压氧组，每组30只。采用改良Allen法建立大鼠脊髓损伤模型。分别于造模前、造模后1天、3天、1周、2周、3周、4周采用BBB评分、斜面测试和改进Tarlov评分对运动功能进行评估。造模后3 d，采用TUNEL法检测实验组脊髓损伤区神经元细胞凋亡情况；采用RT-PCR和Western blot检测脊髓损伤及周围组织中MMP9/2基因及蛋白的表达。造模后4周，HE染色观察脊髓损伤组织病理学形态学改变；采用氟金逆行示踪法观察脊髓神经纤维的再生和分布，透射电镜观察轴突再生情况。高压氧组在治疗2周后各时间点的三项运动功能评分均较脊髓损伤组显著升高（P < 0.05）。造模后第3 d，高压氧组大鼠细胞凋亡指数显著低于脊髓损伤组（P < 0.05）。造模后72 h，与脊髓损伤组比较，高压氧组MMP9/2基因及蛋白表达均显著降低（P < 0.05）。造模后第4周，假手术组氟金阳性神经纤维最多，高压氧组次之，脊髓损伤组次之；各组间差异均有统计学意义（P < 0.05）。透射电镜下，假手术组和高压氧组正中横断面可见新生的无髓神经纤维和有髓神经纤维；高压氧组无髓和有髓神经纤维均多于脊髓损伤组。高压氧治疗通过减少脊髓损伤大鼠神经细胞凋亡和MMP9/2基因及蛋白的表达，对脊髓损伤有保护作用。
To study the effect of hyperbaric oxygen intervention on the microenvironment of nerve regeneration after spinal cord injury modeling and to explore the possible mechanism of nerve regeneration and functional recovery in rats with spinal cord injury. In 98 adult female SD rats, 90 successful models were obtained, which were divided into sham group, spinal cord injury group and hyperbaric oxygen group using randomized block method, 30/group. Spinal cord injury rat model was established in accordance with the modified Allen method. Motor function was assessed at the time points of before modeling, one day, three days, one week, two weeks, three weeks and four weeks after modeling respectively by BBB rating, inclined plane test and improved Tarlov score. At 3 days after modeling, apoptosis of neuronal cells in spinal cord injury region in experimental group was detected by TUNEL method; gene and protein expression of MMP9/2 in spinal cord injury and surrounding tissues was detected by RT-PCR and Western blot assay. At 4 weeks after modeling, histopathological morphological changes in spinal cord injury were observed by HE staining; fluorogold retrograde tracing was used to observe the regeneration and distribution of spinal cord nerve fibers and axon regeneration was observed by TEM. The three motor function scores in hyperbaric oxygen group at each time point after two weeks of treatment were significantly increased compared with spinal cord injury group (P < 0.05). At 3 d after modeling, apoptosis index in hyperbaric oxygen group were significantly lower than those in spinal cord injury group (P < 0.05). At 72 h after modeling, compared with spinal cord injury group, MMP9/2 gene and protein expression in hyperbaric oxygen group was significantly lower (P < 0.05). At four weeks after modeling, fluorogold positive nerve fibers were the most sham group, followed by hyperbaric oxygen group and spinal cord injury group in order; the differences among the groups were statistically significant (P < 0.05). Under TEM, newborn unmyelinated and myelinated nerve fibers could be observed in the middle cross-section in the sham group and hyperbaric oxygen group; unmyelinated and myelinated nerve fibers in hyperbaric oxygen group were more than those in spinal cord injury group. Hyperbaric oxygen therapy played a protective effect on spinal cord injury through reducing apoptosis of neuronal cells and expression of MMP9/2 gene and protein in rats with spinal cord injury.