Studies on alkaloids binding to GC-rich human survivin promoter DNA using positive and negative ion electrospray ionization mass spectrometry.
Studies on alkaloids binding to GC-rich human survivin promoter DNA using positive and negative ion electrospray ionization mass spectrometry.
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使用正负离子电喷雾电离质谱法研究生物碱与富含 GC 的人生存素启动子 DNA 的结合。
DOI：
10.1002/jms.1320
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Shuying Liu
中科院分区：
文献类型：
--
作者：
Zhaofu Wang;Xinhua Guo;Zhiqiang Liu;M. Cui;F. Song;Shuying Liu
利用电喷雾质谱（ESI-MS）研究了13种生物碱与人Survivin启动子关键序列--两个富含GC的DNA双链体的结合。首次应用负离子ESI-MS筛选生物碱与双链体的结合。6种生物碱（包括小檗碱、药根碱、巴马汀、利血平、小檗胺和粉防己碱）显示与靶DNA序列的络合作用。相对结合亲和力估计从负离子的ESI数据，生物碱显示出结合偏好的双链体具有较高的GC含量。记录了配合物的正离子ESI质谱，并与负离子模式下的质谱进行了比较。只有1：1与小檗胺的复合物在正离子质谱中观察到较低的丰度，而与其他生物碱的复合物则完全不存在。碰撞诱导解离（CID）实验表明，与原小檗碱生物碱（小檗碱，药根碱，巴马汀）的复合物解离通过碱损失和共价断裂。相反，与生物碱利血平，小檗胺，粉防己碱的复合物的产物离子光谱显示中性生物碱分子的主要损失，伴随着碱损失和共价裂解在较小程度上。与传统的DNA粘合剂的配合物的气相行为的比较表明，这些生物碱的目标DNA双链体的插入结合模式。
Electrospray ionization mass spectrometry (ESI-MS) was used to investigate the binding of 13 alkaloids to two GC-rich DNA duplexes which are critical sequences in human survivin promoter. Negative ion ESI-MS was first applied to screen the binding of the alkaloids to the duplexes. Six alkaloids (including berberine, jatrorrhizine, palmatine, reserpine, berbamine, and tetrandrine) show complexation with the target DNA sequences. Relative binding affinities were estimated from the negative ion ESI data, and the alkaloids show a binding preference to the duplex with higher GC content. Positive ion ESI mass spectra of the complexes were also recorded and compared with those obtained in negative ion mode. Only the 1 : 1 complex with berbamine was observed with lower abundance in the positive ion mass spectrum while complexes with the other alkaloids were absolutely absent. Collision-induced dissociation (CID) experiments indicate that the complexes with the protoberberine alkaloids (berberine, jatrorrhizine, and palmatine) dissociate via base loss and covalent cleavage. In contrast, product ion spectra of the complexes with the alkaloids reserpine, berbamine, and tetrandrine show the predominant loss of a neutral alkaloid molecule, accompanied by base loss and covalent cleavage to a lesser extent. A comparison of the gas-phase behaviors of complexes with the alkaloids to those with the traditional DNA binders has suggested an intercalative binding mode of these alkaloids to the target DNA duplexes.
DOI：
10.1042/bj3440305
发表时间：
1999-12
期刊：
The Biochemical journal
影响因子：
--
作者：
Fengzhi Li;D. Altieri
通讯作者：
Fengzhi Li;D. Altieri
影响因子：
2.9
作者：
Pilch, DS;Yu, C;Liu, LF
通讯作者：
Liu, LF