Effects of Cold Stimulation on Mitochondrial Activity and VEGF Expression in vitro
Effects of Cold Stimulation on Mitochondrial Activity and VEGF Expression in vitro
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DOI：
10.1055/s-0042-102659
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发表时间：
2016-04
影响因子：
2.5
通讯作者：
T. Sugasawa;N. Mukai;K. Tamura;T. Tamba;S. Mori;Y. Miyashiro;M. Yamaguchi;S. Nissato;Sg Ra;Y. Yoshida;M. Hoshino;H. Ohmori;Y. Kawakami;K. Takekoshi
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文献类型：
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作者：
T. Sugasawa;N. Mukai;K. Tamura;T. Tamba;S. Mori;Y. Miyashiro;M. Yamaguchi;S. Nissato;Sg Ra;Y. Yoshida;M. Hoshino;H. Ohmori;Y. Kawakami;K. Takekoshi
摘要本研究旨在阐明不同温度冷刺激对体外培养的大鼠心肌线粒体活性和血管内皮生长因子（VEGF）表达的影响。使用人成纤维细胞、人间充质干细胞和大鼠骨骼肌成肌细胞系。对于每种细胞类型，将细胞分成4组，并通过放置在碎冰上或漂浮在设定在每个温度下的冷水上，在各种低温（0、4、17和25°C）下刺激3次，每次15分钟。将对照细胞置于37°C的温水中。在最后一次冷刺激后24 h分析与线粒体活性、线粒体DNA拷贝数和VEGF表达相关的因素。在所有细胞类型中，与对照细胞相比，在4°C和17° C刺激的细胞中观察到与线粒体活性和线粒体DNA拷贝数相关的因子显著增加。在4°C刺激的大鼠骨骼肌细胞中，与对照细胞相比，VEGF表达显著增加。我们的数据表明，在一定温度下的冷刺激促进线粒体活性，生物合成和VEGF表达。
Abstract We aimed to clarify the effects of cold stimulation at various temperatures on mitochondrial activity and vascular endothelial growth factor (VEGF) expression in vitro. Human fibroblast, human mesenchymal stem cell, and rat skeletal muscle myoblast cell lines were used. For each cell type, cells were divided into 4 groups and stimulated in various cold temperatures (0, 4, 17 and 25°C) 3 times for 15 min each by placement on crushed ice or floating on cold water set at each temperature. Control cells were subjected to warm water at 37°C. Factors related to mitochondrial activity, mitochondrial DNA copy numbers, and VEGF expression were analyzed 24 h after the last cold stimulation. In all cell types, significant increases of factors related to mitochondrial activity and mitochondrial DNA copy numbers were seen in the 4°C and 17°C-stimulated cells compared with control cells. In rat skeletal muscle cells stimulated at 4°C, VEGF expression significantly increased compared to the control cells. Our data suggest that cold stimulation at certain temperatures promotes mitochondrial activity, biogenesis and VEGF expression.