Seasonal Dynamics of the Airborne Bacterial Community and Selected Viruses in a Children's Daycare Center.
Seasonal Dynamics of the Airborne Bacterial Community and Selected Viruses in a Children's Daycare Center.
复制标题
DOI：
10.1371/journal.pone.0151004
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Marr LC
中科院分区：
文献类型：
--
作者：
Prussin AJ 2nd;Vikram A;Bibby KJ;Marr LC
儿童日托中心似乎是呼吸道传染病传播的枢纽，但关于日托中心存在的空气微生物群落的信息有限。我们调查了日托中心空气中的微生物群落，包括细菌群落的季节动态和特定病毒病原体的存在。在一年的时间里，我们每两周从一家日托中心的暖通空调(HVAC)系统中收集过滤器。16S rRNA基因的扩增和测序显示，空气中主要由变形杆菌、菲米库特、放线杆菌和拟杆菌组成，这些细菌通常与人类皮肤的菌群有关。微生物群落的季节差异不明显；然而，当日托中心关闭并无人居住13天时，群落结构不同。这些结果表明，人类居住，而不是环境，是塑造日托中心空气中微生物群落结构的主要驱动力。使用靶向病毒的聚合酶链式反应，我们检测到呼吸道合胞病毒存在的季节性模式，包括与病毒相关的疾病的典型发生时期；然而，我们在任何时候都没有检测腺病毒或轮状病毒的存在。
Children’s daycare centers appear to be hubs of respiratory infectious disease transmission, yet there is only limited information about the airborne microbial communities that are present in daycare centers. We have investigated the microbial community of the air in a daycare center, including seasonal dynamics in the bacterial community and the presence of specific viral pathogens. We collected filters from the heating, ventilation, and air conditioning (HVAC) system of a daycare center every two weeks over the course of a year. Amplifying and sequencing the 16S rRNA gene revealed that the air was dominated by Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes that are commonly associated with the human skin flora. Clear seasonal differences in the microbial community were not evident; however, the community structure differed when the daycare center was closed and unoccupied for a 13-day period. These results suggest that human occupancy, rather than the environment, is the major driver in shaping the microbial community structure in the air of the daycare center. Using PCR for targeted viruses, we detected a seasonal pattern in the presence of respiratory syncytial virus that included the period of typical occurrence of the disease related to the virus; however, we did not detect the presence of adenovirus or rotavirus at any time.