A Nanoelectronic Enzyme-Linked Immunosorbent Assay for Detection of Proteins in Physiological Solutions
A Nanoelectronic Enzyme-Linked Immunosorbent Assay for Detection of Proteins in Physiological Solutions
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DOI：
10.1002/smll.200901551
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发表时间：
2010-01-18
期刊：
影响因子：
13.3
通讯作者：
Fahmy, Tarek M.
中科院分区：
文献类型：
--
作者：
Stern, Eric;Vacic, Aleksandar;Fahmy, Tarek M.
半导体纳米线是非常有前途的超灵敏、无标记的小分子、DNA、蛋白质和细胞功能传感器。纳米线场效应晶体管（FET）通过感测结合分子的电荷来起作用。然而，生理离子强度的溶液由于离子（德拜）筛选而损害特异性结合事件的检测。一个通用的解决方案，这种限制与开发的混合纳米电子酶联免疫吸附测定（ne-ELISA）相结合的结合底物的酶促转化的电力与电子检测证明。这种新的配置产生了一个本地酶介导的pH值的变化成比例的结合配体浓度。结果表明，纳米线FET配置为pH传感器，可用于定量检测白细胞介素-2在生理缓冲溶液中的浓度低至1.6 pg mL（-1）。通过成功绕过生理液体中固有的德拜筛选，ne-ELISA有望在一系列相关解决方案中广泛适用于配体检测。
Semiconducting nanowires are promising ultrasensitive, label-free sensors for small molecules, DNA, proteins, and cellular function. Nanowire field-effect transistors (FETs) function by sensing the charge of a bound molecule. However, solutions of physiological ionic strength compromise the detection of specific binding events due to ionic (Debye) screening. A general solution to this limitation with the development of a hybrid nanoelectronic enzyme-linked immunosorbent assay (ne-ELISA) that combines the power of enzymatic conversion of a bound substrate with electronic detection is demonstrated. This novel configuration produces a local enzyme-mediated pH change proportional to the bound ligand concentration. It is shown that nanowire FETs configured as pH sensors can be used for the quantitative detection of interleukin-2 in physiologically buffered solution at concentrations as low as 1.6 pg mL(-1). By successfully bypassing the Debye screening inherent in physiological fluids, the ne-ELISA promises wide applicability for ligand detection in a range of relevant solutions.