Characterization of Isomeric Glycans by Reversed Phase Liquid Chromatography-Electronic Excitation Dissociation Tandem Mass Spectrometry.
Characterization of Isomeric Glycans by Reversed Phase Liquid Chromatography-Electronic Excitation Dissociation Tandem Mass Spectrometry.
复制标题
通过反相液相色谱-电子激发解离串联质谱法表征异构聚糖。
DOI：
10.1007/s13361-018-1943-9
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发表时间：
2018
影响因子：
3.2
通讯作者：
Lin,Cheng
中科院分区：
文献类型：
--
作者：
Tang,Yang;Wei,Juan;Costello,CatherineE;Lin,Cheng
生物来源的聚糖中存在大量结构异构体，这对结构糖组学提出了严峻的挑战。异构体结构之间的细微差异要求分析方法能够提供结构细节，同时与在线聚糖分离方法有效配合。虽然液相色谱-串联质谱（LC-MS/MS）是混合物分析的有力工具，但常用的碰撞诱导解离（CID）方法通常不能在MS 2水平产生足够数量的碎片以进行全面的结构表征。在这里，我们研究了金属加合物，全甲基化聚糖的电子激发解离（EED）行为，并确定了关键的光谱特征，可以促进拓扑结构和连接的决定。我们开发了一种基于EED的纳米级反相（RP）LC-MS/MS平台，并证明了其在单一LC-MS/MS分析中实现多达五种结构异构体的完整结构解析的能力。
The occurrence of numerous structural isomers in glycans from biological sources presents a severe challenge for structural glycomics. The subtle differences among isomeric structures demand analytical methods that can provide structural details while working efficiently with on-line glycan separation methods. Although liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful tool for mixture analysis, the commonly utilized collision-induced dissociation (CID) method often does not generate a sufficient number of fragments at the MS2level for comprehensive structural characterization. Here, we studied the electronic excitation dissociation (EED) behaviors of metal-adducted, permethylated glycans, and identified key spectral features that could facilitate both topology and linkage determinations. We developed an EED-based, nanoscale, reversed phase (RP)LC-MS/MS platform, and demonstrated its ability to achieve complete structural elucidation of up to five structural isomers in a single LC-MS/MS analysis.