AMPLIFICATION OF A NOVEL V-ERBB-RELATED GENE IN A HUMAN MAMMARY-CARCINOMA
AMPLIFICATION OF A NOVEL V-ERBB-RELATED GENE IN A HUMAN MAMMARY-CARCINOMA
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DOI：
10.1126/science.2992089
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
AARONSON, SA
中科院分区：
文献类型：
--
作者：
KING, CR;KRAUS, MH;AARONSON, SA
编码表皮生长因子（EGF）受体的细胞基因与禽成红细胞增多症病毒的癌基因具有相当大的同源性。在人类乳腺癌中，鉴定出与v-erbB相关的DNA序列，但其扩增方式似乎将其与EGF受体基因区分开来。该DNA片段的分子克隆和核苷酸序列分析表明，该DNA片段中存在两个推定的外显子，其预测的氨基酸序列与erbB/EGF受体的相应序列密切相关，但不同。此外，该DNA片段鉴定了与EGF受体基因的转录物不同的5-羟色胺酶转录物。因此，酪氨酸激酶原癌基因家族的一个新成员已被确定在其扩增的基础上，在人类乳腺癌。
The cellular gene encoding the receptor for epidermal growth factor (EGF) has considerable homology to the oncogene of avian erythroblastosis virus. In a human mammary carcinoma, a DNA sequence was identified that is related to v-erbB but amplified in a manner that appeared to distinguish it from the gene for the EGF receptor. Molecular cloning of this DNA segment and nucleotide sequence analysis revealed the presence of two putative exons in a DNA segment whose predicted amino acid sequence was closely related to, but different from, the corresponding sequence of theerbB/EGF receptor. Moreover, this DNA segment identified a 5-kilobase transcript distinct from the transcripts of the EGF receptor gene. Thus, a new member of the tyrosine kinase proto-oncogene family has been identified on the basis of its amplification in a human mammary carcinoma.