Neuropeptides and neurotrophins in neonatal blood of children with autism or mental retardation
Neuropeptides and neurotrophins in neonatal blood of children with autism or mental retardation
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DOI：
10.1002/ana.1024
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发表时间：
2001-05-01
影响因子：
11.2
通讯作者：
Phillips, TM
中科院分区：
文献类型：
--
作者：
Nelson, KB;Grether, JK;Phillips, TM
很少有人探索自闭症大脑发育的主要生物调节因子。在自闭症谱系障碍（n = 69）、无自闭症的精神发育迟滞（n = 60）或脑瘫儿童的存档新生儿血液中采用循环免疫亲和层析法测定CP组63例和对照组54例脑内神经肽P物质（SP）、血管活性肠肽（VIP）、垂体腺苷酸环化酶激活肽（PACAP）、降钙素基因相关肽（CGRP）、神经生长因子（NGF）、脑源性神经营养因子（BDNF）、神经营养因子3（NT 3）和神经营养因子4/5（NT 4/5）、VIP、CGRP、BDNF和NT 4/5的浓度均高于对照组（ANOVA，成对差异的Scheffe检验的所有p值< 0.0001）在自闭症谱系的儿童和那些没有自闭症的精神发育迟滞儿童中比在对照组儿童中。在99%的自闭症儿童和97%的智力迟钝儿童中，至少有一种物质的水平超过了所有对照儿童。在自闭症谱系亚组（核心综合征伴或不伴精神发育迟滞，其他自闭症谱系障碍伴或不伴精神发育迟滞）以及存在或不存在退化史的情况下，浓度相似。在患有精神发育迟滞的儿童中，浓度没有因严重程度或已知原因而不同（n = 11，包括4例唐氏综合征）。与对照组相比，CP儿童中测得的物质浓度相似。SP、PACAP、NGF和NT 3在诊断组间无差异。没有测量的分析物区分自闭症儿童与单独的智力迟钝儿童。在自闭症和认知功能障碍的异质组中，在生命的第一天抽取的外周血中观察到某些神经肽和神经营养因子的过度表达。
There has been little exploration of major biologic regulators of cerebral development in autism. In archived neonatal blood of children with autistic spectrum disorders (n = 69), mental retardation without autism (n = 60), or cerebral palsy (CP, n = 63) and of control children (n = 54), we used recycling immunoaffinity chromatography to measure the neuropeptides substance P (SP), vasoactive intestinal peptide (VIP), pituitary adenylate cyclase-activating polypeptide (PACAP), calcitonin gene-related peptide (CGRP), and the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT3), and neurotrophin 4/5 (NT4/5), Neonatal concentrations of VIP, CGRP, BDNF, and NT4/5 were higher (ANOVA, all p values < 0.0001 by Scheffe test for pairwise differences) in children in the autistic spectrum and in those with mental retardation without autism than in control children. In 99% of children with autism and 97% with mental retardation, levels of at least one of these substances exceeded those of all control children. Concentrations were similar in subgroups of the autistic spectrum (core syndrome with or without mental retardation, other autistic spectrum disorders with or without mental retardation) and in the presence or absence of a history of regression. Among children with mental retardation, concentrations did not differ by severity or known cause (n = 11, including 4 with Down syndrome). Concentrations of measured substances were similar in children with CP as compared with control subjects. SP, PACAP, NGF, and NT3 were not different by diagnostic group. No measured analyte distinguished children with autism from children with mental retardation alone. In autism and in a heterogeneous group of disorders of cognitive function, overexpression of certain neuropeptides and neurotrophins was observed in peripheral blood drawn in the first days of life.