Synthesis of double-clickable functionalised graphene oxide for biological applications.
Synthesis of double-clickable functionalised graphene oxide for biological applications.
复制标题
DOI：
10.1039/c5cc05412e
复制
发表时间：
2015-10-18
期刊：
影响因子：
--
通讯作者：
Al-Jamal KT
中科院分区：
文献类型：
--
作者：
Mei KC;Rubio N;Costa PM;Kafa H;Abbate V;Festy F;Bansal SS;Hider RC;Al-Jamal KT
合成了叠氮-和炔-双官能化氧化石墨烯（Click 2 GO），并用ATR-FTIR、TGA和拉曼光谱对其进行了表征。合成了叠氮-和炔-双官能化氧化石墨烯（Click 2 GO），并利用衰减全反射傅里叶变换红外光谱（ATR-FTIR）、热重分析（TGA）和拉曼光谱对其进行了表征。用间氯过氧苯甲酸（mCPBA）预处理GO后，发现叠氮化物含量增加了14个百分点，用元素分析法测定。使用改良的乳酸脱氢酶（mLDH）测定法测定，在高达100 μg mL-1和孵育72 h时，未发现对A549细胞活力的影响。进行两个连续的铜⑴催化的叠氮化物-炔环加成（CuAAC）反应以将炔丙基修饰的血脑屏障靶向肽Angiopep-2和双叠氮化物聚乙二醇（MW = 3500）缀合至Click 2 GO。用ATR-FTIR和TGA表征最终缀合物。
Azide- and alkyne-double functionalised graphene oxide (Click2 GO) was synthesised and characterised with ATR-FTIR, TGA, and Raman spectroscopy. Azide- and alkyne-double functionalised graphene oxide (Click2 GO) was synthesised and characterised with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA) and Raman spectroscopy. Fourteen-percentage increase in azide content was found, after pre-treatment of GO with meta-chloroperoxybenzoic acid (mCPBA), determined with elemental analysis. No effect on A549 cell viability was found, up to 100 μg mL–1 and 72 h of incubation, determined with the modified lactate dehydrogenase (mLDH) assay. Two sequential copper(i) catalysed azide–alkyne cycloaddition (CuAAC) reactions were performed to conjugate the propargyl-modified blood–brain barrier targeting peptide Angiopep-2, and a bis-azide polyethylene glycol (M W = 3500), to the Click2 GO. The final conjugate was characterised with ATR-FTIR and TGA.