ANTIBODY-DEPENDENT CELL-MEDIATED CYTO-TOXICITY AGAINST INFLUENZA-VIRUS INFECTED-CELLS
ANTIBODY-DEPENDENT CELL-MEDIATED CYTO-TOXICITY AGAINST INFLUENZA-VIRUS INFECTED-CELLS
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DOI：
10.1093/infdis/148.5.785
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发表时间：
1983-01-01
影响因子：
6.4
通讯作者：
KARZON, DT
中科院分区：
文献类型：
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作者：
HASHIMOTO, G;WRIGHT, PF;KARZON, DT
人外周血中的3种主要免疫活性细胞（淋巴细胞、中性粒细胞和单核细胞）被证明是体外抗流感病毒感染的幼仓鼠肾细胞的抗体依赖性细胞介导的细胞毒性（ADCC）的效应细胞。淋巴细胞的细胞毒性是由携带FcIgG受体的空细胞和T γ介导的。[IgG包含]细胞。这些效应群体最好由HNK-1（一种针对人自然杀伤细胞和ADCC介导细胞的单克隆抗体）定义。抗体负责ADCC对流感病毒感染的细胞检测后，自然感染和接种灭活和减毒活病毒的幼儿血清。ADCC抗体出现在血凝抑制抗体之前，并在减毒活疫苗接种后至少持续1年。ADCC抗体是亚型特异性的，但在亚型内反应性相当广泛。血凝素和神经氨酸酶是ADCC抗体的抗原决定簇。用异源亚型流感病毒攻击后，观察到对原始毒株的回忆性反应。
The 3 major immunocompetent cells in human peripheral blood (lymphocytes, neutrophils and monocytes) were shown to be effector cells for antibody-dependent cell-mediated cytotoxicity (ADCC) against influenza virus-infected baby hamster kidney cells in vitro. Lymphocyte cytotoxicity was mediated by FcIgG receptor-bearing null cells and T.gamma. [IgG containing] cells. These effector populations were best defined by HNK-1, a monoclonal antibody to human natural killer and ADCC-mediator cells. Antibody responsible for ADCC against influenza virus-infected cells was detectable in sera of young children after natural infection and after vaccination with inactivated and live attenuated viruses. ADCC antibody appeared before hemagglutination-inhibiting antibody and persisted for at least 1 yr after vaccination with live attenuated vaccine. ADCC antibody was subtype-specific but quite broadly reactive within a subtype. Both hemagglutinin and neuraminidase were antigenic determinants for ADCC antibody. An anamnestic response to the original strain was observed after challenge with influenza virus of a heterologous subtype.