CENTRAL REGION COMPONENT1, a Novel Synaptonemal Complex Component, Is Essential for Meiotic Recombination Initiation in Rice
CENTRAL REGION COMPONENT1, a Novel Synaptonemal Complex Component, Is Essential for Meiotic Recombination Initiation in Rice
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中央区成分1是一种新型联会复合体成分，对于水稻减数分裂重组启动至关重要
DOI：
10.1105/tpc.113.113175
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发表时间：
2013-08-01
期刊：
影响因子：
11.6
通讯作者：
Cheng, Zhukuan
中科院分区：
文献类型：
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作者：
Miao, Chunbo;Tang, Ding;Cheng, Zhukuan
在减数分裂中，同源重组需要程序性DNA双链断裂（DSB）的形成和联会复合体（SC）的组装与DSB修复偶联。尽管SC在物种间表现出广泛的结构保守性，但它们的组分在序列水平上保守性很差。在这里，我们确定了一个新的SC组件，命名为中央区域组件1（CRC1），在水稻（水稻）。CRC1与水稻SC横丝蛋白ZEP1以相互依赖的方式共定位于SC的中心区域。与这种共定位一致，CRC1在酵母双杂交测定中与ZEP1相互作用。CRC 1与酿酒酵母粗线期检查点2（Pch 2）和小家鼠甲状腺受体相互作用蛋白13（TRIP 13）是同源的，可能是一种保守的SC组分。此外，我们提供的证据表明，CRC1是必不可少的减数分裂DSB的形成。CRC1与水稻减数分裂中同源配对畸变1（PAIR1）在体外相互作用，表明这些蛋白质作为复合物促进DSB形成。PAIR 2是水稻中芽殖酵母同源配对1的直系同源物，是同源染色体配对所必需的。我们发现，CRC1也是必不可少的招聘PAIR2到减数分裂染色体。在此确定的CRC1的作用尚未报告为Pch2或TRIP13。
In meiosis, homologous recombination entails programmed DNA double-strand break (DSB) formation and synaptonemal complex (SC) assembly coupled with the DSB repair. Although SCs display extensive structural conservation among species, their components identified are poorly conserved at the sequence level. Here, we identified a novel SC component, designated CENTRAL REGION COMPONENT1 (CRC1), in rice (Oryza sativa). CRC1 colocalizes with ZEP1, the rice SC transverse filament protein, to the central region of SCs in a mutually dependent fashion. Consistent with this colocalization, CRC1 interacts with ZEP1 in yeast two-hybrid assays. CRC1 is orthologous to Saccharomyces cerevisiae pachytene checkpoint2 (Pch2) and Mus musculus THYROID RECEPTOR-INTERACTING PROTEIN13 (TRIP13) and may be a conserved SC component. Additionally, we provide evidence that CRC1 is essential for meiotic DSB formation. CRC1 interacts with HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 (PAIR1) in vitro, suggesting that these proteins act as a complex to promote DSB formation. PAIR2, the rice ortholog of budding yeast homolog pairing1, is required for homologous chromosome pairing. We found that CRC1 is also essential for the recruitment of PAIR2 onto meiotic chromosomes. The roles of CRC1 identified here have not been reported for Pch2 or TRIP13.