Identification of floral volatiles involved in recognition of oilseed rape flowers, Brassica napus by honeybees, Apis mellifera
Identification of floral volatiles involved in recognition of oilseed rape flowers, Brassica napus by honeybees, Apis mellifera
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DOI：
10.1023/b:joec.0000006446.21160.c1
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发表时间：
1997-07-01
影响因子：
2.3
通讯作者：
Wadhams, LJ
中科院分区：
文献类型：
--
作者：
Blight, MM;LeMetayer, M;Wadhams, LJ
用空气夹带法采集了油菜、甘蓝型油菜和花朵中的挥发物，并通过蜜蜂条件鼻子延伸(CPE)分析证实了它们与花朵自然气味分布的相关性。采用气相色谱-毛细管电泳联用分析方法，确定了油菜花识别中的关键化合物，并用气相色谱-质谱联用法进行了鉴定，并与正品进行了比较。气相色谱图的六个区域引发了先前条件蜜蜂对总提取物的CPE反应，从这些区域鉴定出了16种化合物，当用合成样品测试时，这些化合物引起了条件蜜蜂的CPE活性。16个化合物中有8个反应最好，分别是：Cy-Pinene、苯乙醛、对伞花烃、α-松油烯、芳樟醇、2-苯乙醇、(E，E)-α-法尼烯和3-Carene。当蜜蜂适应于花挥发油的总提取物时，8种成分的混合物引起83%的个体的反应，表明8种成分的混合物占总提取物CPE活性的主要部分。此外，三种最活跃的化合物苯乙醛、芳樟醇和(E，E，)-α-法尼烯的混合物，在后者适应了八组分的混合物后，引起了85%的蜜蜂的反应。因此，这三种化合物似乎在识别八组分混合物以及由此推断的油菜花的识别中发挥了关键作用。
Volatiles from oilseed rape, Brassica napus, flowers were sampled by air entrainment and their relevance to the natural odor profile of the flowers was confirmed by conditioned proboscis extension (CPE) assays with honeybee, Apis mellifera L., foragers. Coupled gas chromatography (GC)-CPE analysis of the air entrainment samples was used to locate key compounds involved in the recognition of B. napus flowers, and the compounds were then identified using coupled gas chromatography-mass spectrometry and comparison with authentic samples. Six regions of the gas chromatograms elicited CPE responses from bees previously conditioned to the total extract, and from these areas 16 compounds were identified that elicited CPE activity from conditioned bees when tested with synthetic samples. Eight of the 16, cy-pinene, phenylacetaldehyde, p-cymene, alpha-terpinene, linalool, 2-phenylethanol, (E,E)-alpha-farnesene, and 3-carene, gave the highest responses. When the bees were conditioned to the total extract of flower volatiles, a mixture of the eight components elicited responses from 83% of the individuals, suggesting that the eight-component mixture accounted for a major part of the CPE activity of the total extract. In addition, a mixture of the three most active compounds, phenylacetaldehyde, linalool, and (E,E,)-alpha-farnesene, evoked responses from 85% of the bees after the latter had been conditioned to the eight-component mixture. Thus, these three compounds appear to play a key role in the recognition of the eight component mixture and, by inference, of oilseed rape flowers.