GENERATION OF HUMORAL IMMUNE-RESPONSES AGAINST HERPES-SIMPLEX VIRUS TYPE-2 IN THE MURINE FEMALE GENITAL-TRACT
GENERATION OF HUMORAL IMMUNE-RESPONSES AGAINST HERPES-SIMPLEX VIRUS TYPE-2 IN THE MURINE FEMALE GENITAL-TRACT
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DOI：
10.1016/s0042-6822(95)80038-7
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发表时间：
1995-01-10
期刊：
影响因子：
3.7
通讯作者：
BERNSTEIN, DI
中科院分区：
文献类型：
--
作者：
MILLIGAN, GN;BERNSTEIN, DI
采用胸苷激酶缺陷（tk(-)）型单纯疱疹病毒2型（HSV-2）感染小鼠生殖器模型，检测局部泌尿生殖道抗体对HSV-2的反应。阴道接种HSV-2 tk（-）可防止随后致命剂量的HSV-2毒株的生殖器攻击。原发阴道感染后，血清和阴道分泌物中主要检测到hsv特异性IgG抗体。初次接种HSV-2 tk（-）后，在生殖器淋巴结（gLN）中首先检测到hsv特异性IgG抗体分泌细胞（ASC），且数量最多。原发HSV-2阴道感染后，泌尿生殖道黏膜未检出hsv特异性IgG或IgA AsC。单纯疱疹病毒免疫小鼠阴道HSV-2攻击导致单纯疱疹病毒特异性血清IgG抗体和阴道IgA抗体滴度升高。攻毒后第4天在gLN中检测hsv特异性IgG ASC，攻毒后第6天和第7天在脾脏和生殖器粘膜中检测hsv特异性IgG ASC。这些结果表明，HSV生殖器感染后的泌尿生殖道体液反应起源于gLN，并且gLN可能是阴道攻击后泌尿生殖道粘膜中存在的HSV特异性IgG和iga分泌细胞的主要来源。(C) 1995学术出版社，Inc。
A murine model of genital infection with a thymidine kinase-deficient (tk(-)) strain of herpes simplex virus type 2 (HSV-2) was utilized to examine the local urogenital antibody response to HSV-2. Vaginal inoculation with HSV-2 tk(-) protected against a subsequent genital challenge with a lethal dose of virulent HSV-2. After primary vaginal infection, predominantly HSV-specific IgG antibodies were detected in serum and vaginal secretions. HSV-specific IgG antibody-secreting cells (ASC) were detected first and in greatest numbers in the genital lymph nodes (gLN) after primary HSV-2 tk(-) inoculation. HSV-specific IgG or IgA AsC were not detected in the urogenital mucosa after primary HSV-2 vaginal infection. Vaginal HSV-2 challenge of HSV-immune mice resulted in increased HSV-specific serum IgG antibody and vaginal IgA antibody titers. HSV-specific IgG ASC were detected by 4 days postchallenge in gLN and by Days 6 and 7 postchallenge in the spleen and genital mucosa. These results suggest that urogenital humoral responses originate in the gLN following HSV genital infection and that gLN may serve as the primary source of the HSV-specific IgG- and IgA-secreting cells present in the urogenital mucosa after vaginal challenge. (C) 1995 Academic Press, Inc.