Drosophila Set1 is the major histone H3 lysine 4 trimethyltransferase with role in transcription
Drosophila Set1 is the major histone H3 lysine 4 trimethyltransferase with role in transcription
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DOI：
10.1038/emboj.2011.194
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发表时间：
2011-07-20
期刊：
影响因子：
11.4
通讯作者：
Kusch, Thomas
中科院分区：
文献类型：
--
作者：
Ardehali, M. Behfar;Mei, Amanda;Kusch, Thomas
组蛋白H3赖氨酸4三甲基化（H3 K4 me 3）是启动子近端组蛋白转录基因的主要标志。在这里，我们报告说，以前未知的果蝇H3 K4甲基转移酶，dSet 1，而不是其他假定的组蛋白H3 K4甲基转移酶（Trithorax; Trithorax相关蛋白），主要负责组蛋白H3 K4三甲基化。功能和蛋白质组学研究表明，dSet 1是一个保守的H3 K4三甲基转移酶复合物和多烯染色和活细胞成像分析显示广泛的关联dSet 1与转录活性基因的组成部分。dSet 1存在于所有测试基因的启动子区，包括活化的Hsp 70和Hsp 26热休克基因，并且是测试基因的最佳mRNA积累所需的。在热休克蛋白70的情况下，mRNA生产缺陷dSet 1 RNAi处理的细胞伴随着保留Pol II在启动子。我们的数据表明，dSet 1依赖H3 K4 me 3是负责在活性启动子，确保最佳的Pol II释放到生产性伸长的染色质结构的产生。The EMBO Journal（2011）30，2817-2828. doi：10.1038/daj.2011.194; 2011年6月21日在线发布
Histone H3 lysine 4 trimethylation (H3K4me3) is a major hallmark of promoter-proximal histones at transcribed genes. Here, we report that a previously uncharacterized Drosophila H3K4 methyltransferase, dSet1, and not the other putative histone H3K4 methyltransferases (Trithorax; Trithorax-related protein), is predominantly responsible for histone H3K4 trimethylation. Functional and proteomics studies reveal that dSet1 is a component of a conserved H3K4 trimethyltransferase complex and polytene staining and live cell imaging assays show widespread association of dSet1 with transcriptionally active genes. dSet1 is present at the promoter region of all tested genes, including activated Hsp70 and Hsp26 heat shock genes and is required for optimal mRNA accumulation from the tested genes. In the case of Hsp70, the mRNA production defect in dSet1 RNAi-treated cells is accompanied by retention of Pol II at promoters. Our data suggest that dSet1-dependent H3K4me3 is responsible for the generation of a chromatin structure at active promoters that ensures optimal Pol II release into productive elongation. The EMBO Journal ( 2011) 30, 2817-2828. doi:10.1038/emboj.2011.194; Published online 21 June 2011