Mobilisation of enterocyte fat stores by oral glucose in humans
Mobilisation of enterocyte fat stores by oral glucose in humans
复制标题
DOI：
10.1136/gut.52.6.834
复制
发表时间：
2003-06-01
期刊：
影响因子：
24.5
通讯作者：
Frayn, KN
中科院分区：
文献类型：
--
作者：
Robertson, MD;Parkes, M;Frayn, KN
背景和目标：当高脂肪口服负荷后几小时进一步摄入营养物质时，血浆三酰甘油（TG）在餐后早期出现峰值。本研究的目的是调查的位置和释放的脂质从胃肠道。方法：10名健康患者接受食管胃内窥镜检查（OGD）。在t=0时，所有患者摄入50 g脂肪乳剂，在t=5小时时，他们摄入水或38 g葡萄糖溶液。在t=6小时时进行OGD，并评价空肠活检样品的脂肪储存。一个亚组的五个科目，然后进行了平行的代谢研究，在同一个两餐protocol.Results：口服脂肪后，在t=0，随后摄入葡萄糖的患者的样本表现出显着减少染色的粘膜和粘膜下层的空肠内的脂质比明显的患者只消耗水（p=0.028）。口服葡萄糖后，肠上皮细胞细胞质内的脂质储存也较少（p=0.005）。在代谢研究中，口服葡萄糖消耗5小时后，口服脂肪导致餐后血浆TG，乳糜微粒-TG，载脂蛋白B48浓度的峰值相比，口服water.Conclusion：脂肪负荷后，脂肪被保留在空肠组织内，并释放到血浆葡萄糖摄入后，导致乳糜微粒-TG的峰值，这已牵连到动脉粥样硬化的发病机制。
Background and aims: When a high fat oral load is followed several hours later by further ingestion of nutrients, there is an early postprandial peak in plasma triacylglycerol (TG). The aim of this study was to investigate the location and release of lipid from within the gastrointestinal tract.Methods: Ten healthy patients undergoing oesopho-gastro-duodenoscopy (OGD) were recruited. At t=0, all patients consumed a 50 g fat emulsion and at t=5 hours they consumed either water or a 38 g glucose solution. OGD was performed at t=6 hours and jejunal biopsy samples were evaluated for fat storage. A subgroup of five subjects then underwent a parallel metabolic study in which postprandial lipid and hormone measurements were taken during an identical two meal protocol.Results: Following oral fat at t=0, samples from patients that had subsequently ingested glucose exhibited significantly less staining for lipid within the mucosa and submucosa of the jejunum than was evident in patients that had consumed only water (p=0.028). There was also less lipid storage within the cytoplasm of enterocytes (p=0.005) following oral glucose. During the metabolic study, oral glucose consumed five hours after oral fat resulted in a postprandial peak in plasma TG, chylomicron-TG, and apolipoprotein B48 concentration compared with oral water.Conclusion: After a fat load, fat is retained within the jejunal tissue and released into plasma following glucose ingestion, resulting in a peak in chylomicron-TG which has been implicated in the pathogenesis of atherosclerosis.