Ribosomal protein methyltransferases in the yeast Saccharomyces cerevisiae: Roles in ribosome biogenesis and translation
Ribosomal protein methyltransferases in the yeast Saccharomyces cerevisiae: Roles in ribosome biogenesis and translation
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DOI：
10.1016/j.bbrc.2016.01.107
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发表时间：
2016-02-12
影响因子：
3.1
通讯作者：
Clarke, Steven
中科院分区：
文献类型：
--
作者：
Al-Hadid, Qais;White, Jonelle;Clarke, Steven
在酿酒酵母和高等真核生物中，甲基转移酶体的显著百分比致力于翻译机器的甲基化。翻译机器的RNA组分的甲基化已经被广泛研究，并且对于结构稳定性、核糖体生物发生和翻译保真度是重要的。然而，核糖体蛋白质甲基化的功能影响，其同源甲基转移酶仍然是未知的。以前的工作已经表明，核糖体蛋白Rp 13甲基转移酶，组氨酸蛋白甲基转移酶1（Hpm 1），是重要的核糖体生物合成和翻译延伸保真度。在这项研究中，酵母菌株缺乏的十个核糖体蛋白甲基转移酶在S。酿酒酵母中的核糖体生物合成和翻译的潜在缺陷进行了检查。像Hpm 1缺陷细胞一样，其他9种核糖体蛋白甲基转移酶中有4种的缺失导致核糖体亚基合成缺陷。所有的突变株表现出耐核糖体抑制剂茴香霉素和/或放线菌酮在平板试验，但不是在液体培养。测量终止密码子通读、氨基酸错误掺入和程序化-1核糖体移码的翻译保真度测定显示，十种酶中的八种对于翻译延伸保真度是重要的，其余两种对于翻译终止效率是必需的。总而言之，这些结果表明S.酿酒酵母在核糖体的生物合成和翻译中起重要作用。(C)2016 Elsevier Inc. All rights reserved.
A significant percentage of the methyltransferasome in Saccharomyces cerevisiae and higher eukaryotes is devoted to methylation of the translational machinery. Methylation of the RNA components of the translational machinery has been studied extensively and is important for structure stability, ribosome biogenesis, and translational fidelity. However, the functional effects of ribosomal protein methylation by their cognate methyltransferases are still largely unknown. Previous work has shown that the ribosomal protein Rpl3 methyltransferase, histidine protein methyltransferase 1 (Hpm1), is important for ribosome biogenesis and translation elongation fidelity. In this study, yeast strains deficient in each of the ten ribosomal protein methyltransferases in S. cerevisiae were examined for potential defects in ribosome biogenesis and translation. Like Hpm1-deficient cells, loss of four of the nine other ribosomal protein methyltransferases resulted in defects in ribosomal subunit synthesis. All of the mutant strains exhibited resistance to the ribosome inhibitors anisomycin and/or cycloheximide in plate assays, but not in liquid culture. Translational fidelity assays measuring stop codon readthrough, amino acid misincorporation, and programmed -1 ribosomal frameshifting, revealed that eight of the ten enzymes are important for translation elongation fidelity and the remaining two are necessary for translation termination efficiency. Altogether, these results demonstrate that ribosomal protein methyltransferases in S. cerevisiae play important roles in ribosome biogenesis and translation. (C) 2016 Elsevier Inc. All rights reserved.