Impaired recovery and cytolytic function of CD56+ T and non-T cells in systemic lupus erythematosus following in vitro polyclonal T cell stimulation. Studies in unselected patients and monozygotic disease-discordant twins.
Impaired recovery and cytolytic function of CD56+ T and non-T cells in systemic lupus erythematosus following in vitro polyclonal T cell stimulation. Studies in unselected patients and monozygotic disease-discordant twins.
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体外多克隆 T 细胞刺激后系统性红斑狼疮中 CD56 T 和非 T 细胞的恢复和溶细胞功能受损。
DOI：
10.1002/art.1780391110
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发表时间：
1996
影响因子：
--
通讯作者：
Horwitz,DA
中科院分区：
文献类型：
--
作者：
Stohl,W;Elliott,JE;Hamilton,AS;Deapen,DM;Mack,TM;Horwitz,DA
Objective. To determine whether there is impaired generation and cytolytic function of CD56+ T cells and non‐T cells in human systemic lupus erythematosus (SLE).Methods. Peripheral blood mononuclear cells (PBMC) were obtained from 73 patients with SLE, 39 normal controls, and 9 pairs of monozygotic (MZ) twins discordant for SLE. PBMC were stimulated with anti‐CD3 monoclonal antibody, maintained in interleukin‐2, and assayed for percentages of total CD56+ cells and CD56+ T cells by flow cytometry, and for cytolytic activity against51Cr‐labeled Daudi target cells.Results. Despite normal total cell expansion, the percentages of recovered CD56+ T cells and total CD56+ cells were 1.6‐fold and 1.8‐fold lower, respectively, in patients with SLE compared with normal controls (P= 0.011 andP< 0.001, respectively). Cytolytic activities of isolated total CD56+ cells and CD56+ T cells and were also reduced in patients with SLE compared with normal controls (P= 0.033). These defects associated with SLE were independent of disease activity and immunosuppressive medications, and they reflected impaired maturation of cytolytic effector cells rather than a deficiency in precursor cell number. In MZ twins discordant for SLE, recovered percentages of CD56+ cells and cytolytic responses were very low in 4 of 8 and 6 of 9 co‐twins with SLE, respectively. Cell‐mixing experiments with the PBMC of the MZ twins demonstrated that the E+ cell fractions (containing all T cells and CD56+ non‐T cells) from the co‐twins with SLE had decreased ability to generate cytolytic activity compared with the corresponding E+ cell fractions from the healthy co‐twins. However, recovered percentages of CD56+ cells and non‐T cells and cytolytic responses were also depressed in 4 of 8 and 4 of 9 healthy co‐twins, respectively.Conclusion. Impaired CD56+ T cell and non‐T cell responses are a feature of SLE and may antedate the onset of clinical disease.
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尽管系统性红斑狼疮中多克隆 T 细胞增殖正常，但多克隆 T 细胞介导的细胞溶解活性的产生受损。
DOI：
--
发表时间：
1992
期刊：
Clinical Immunology and Immunopathology
影响因子：
--
作者：
W. Stohl
通讯作者：
W. Stohl
用抗 CD3 单克隆抗体与 IL2 刺激人外周血单核细胞：尽管对产生不受限制的细胞溶解活性有相似的影响，但对 T 细胞依赖性 B 细胞分化的影响不同。
DOI：
10.1016/0090-1229(92)90244-i
发表时间：
1992
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
W. Stohl;J. Elliott
通讯作者：
J. Elliott
影响因子：
--
作者：
RICHARDSON, B;SCHEINBART, L;JOHNSON, M
通讯作者：
JOHNSON, M
系统性红斑狼疮中自然杀伤细胞活性异常：裂解事件的内在缺陷。
影响因子：
4.4
作者：
P. Katz;A. Zaytoun;J. Lee;R. Panush;S. Longley
通讯作者：
S. Longley
通过抗 CD3 单克隆抗体诱导 T 细胞依赖性 B 细胞分化。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Stohl,W;Posnett,DN;Chiorazzi,N
通讯作者：
Chiorazzi,N