Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step
Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step
复制标题
DOI：
10.1016/j.foodchem.2014.02.112
复制
发表时间：
2014-09-01
期刊：
影响因子：
8.8
通讯作者：
Jeon, You-Jin
中科院分区：
文献类型：
--
作者：
Lee, Ji-Hyeok;Ko, Ju-Young;Jeon, You-Jin
d Ecklonia cave的各种生物活性根皮单宁（例如，dieckol、eckol、6，6-bieckol、间苯三酚、间苯三酚和间苯岩藻呋喃酚-A）。然而，它们的分离和纯化并不容易。离心分配色谱法（CPC）可以有效地从大肠杆菌中分离纯化各种生物活性物质。腔静脉成功地从E.通过CPC用包含正己烷：EtOAc：甲醇：水（2：7：3：7，v/v）溶液的两相溶剂系统进行cava。通过一步CPC系统从粗提取物（500 mg）中分离出柴油（级分1，40.2 mg）、间苯三酚呋喃酚-A（级分III，31.1 mg）和级分11（34.1 mg）以及2，7-间苯三酚-6，6-柴油和邻苯三酚-间苯三酚-6，6-柴油。根据高效液相色谱（HPLC）和电喷雾电离多级串联质谱分析，分离的狄可酚和间苯呋喃甲酸酯-A的纯度>= 90%。通过反相HPLC从级分II收集纯化的2，7-间苯三酚-6，6-bieckol和焦性没食子酚-间苯三酚-6，6-bieckol。因此，CPC系统可用于从E.腔静脉(C)2014爱思唯尔有限公司版权所有。
d Various bioactive phlorotannins of Ecklonia cave (e.g., dieckol, eckol, 6,6-bieckol, phloroglucinol, phloroeckol, and phlorofucofuroeckol-A) are reported. However, their isolation and purification are not easy. Centrifugal partition chromatography (CPC) can be used to efficiently purify the various bioactive-compounds efficiently from E. cava. Phlorotannins are successfully isolated from the ethyl acetate (EtOAc) fraction of E. cava by CPC with a two-phase solvent system comprising n-hexane:EtOAc:methanol:water (2:7:3:7, v/v) solution. The dieckol (fraction 1, 40.2 mg), phlorofucofuroeckol-A (fraction III, 31.1 mg), and fraction 11 (34.1 mg) with 2,7-phloroglucino1-6,6-bieckol and pyrogallol-phloroglucino1-6,6-bieckol are isolated from the crude extract (500 mg) by a one-step CPC system. The purities of the isolated dieckol and phlorofucofuroeckol-A are >= 90% according to high performance liquid chromatography (HPLC) and electrospray ionization multi stage tandem mass spectrometry analyses. The purified 2,7-phloroglucino1-6,6-bieckol and pyrogallol-phloroglucino1-6,6-bieckol are collected from fraction II by recycle-HPLC. Thus, the CPC system is useful for easy and simple isolation of phlorotannins from E. cava. (C) 2014 Elsevier Ltd. All rights reserved.