Over-expression of FoxM1 stimulates cyclin B1 expression
Over-expression of FoxM1 stimulates cyclin B1 expression
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DOI：
10.1016/s0014-5793(01)02915-5
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发表时间：
2001-10-19
期刊：
影响因子：
3.5
通讯作者：
Yao, KM
中科院分区：
文献类型：
--
作者：
Leung, TWC;Lin, SSW;Yao, KM
FoxM 1（以前称为WIN，HFH-11或Trident）是一种在增殖细胞中广泛表达的叉头盒（Fox）转录因子。各种研究结果，包括最近对FoxM 1敲除小鼠的分析，表明FoxM 1是细胞周期进程中正常S-M偶联所必需的。为了研究FoxM 1及其下游调控靶点的调控作用，建立了三个稳定转染的HeLa细胞系，这些细胞系显示强力霉素（dox）诱导的FoxM 1表达。通过dox诱导过表达FoxM 1促进生长从血清饥饿中恢复。使用流式细胞术、Western和北方分析对细胞周期蛋白BI和D1水平进行定量显示，FoxM 1水平升高导致细胞周期蛋白B1的刺激，但不导致细胞周期蛋白DI的表达。在dox-诱导系中和与组成型FoxM 1表达质粒共转染后的瞬时报告基因测定表明FoxM 1可以激活细胞周期蛋白B1启动子。(C)2001年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role of FoxM1 and its downstream regulatory targets, three stably transfected HeLa lines that display doxycycline (dox)-inducible FoxM1 expression were established. Overexpression of FoxM1 by dox induction facilitates growth recovery from serum starvation. Quantitation of cyclin BI and D1 levels using flow cytometric, Western and Northern analyses reveals that elevated FoxM1 levels lead to stimulation of cyclin B1 but not cyclin DI expression. Transient reporter assays in the dox-inducible lines and upon co-transfection with a constitutive FoxM1 expression plasmid suggest that Fox M I can activate the cyclin B1 promoter. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.