Characterization of a gene that is inversely correlated with estrogen receptor expression (ICERE-1) in breast carcinomas
Characterization of a gene that is inversely correlated with estrogen receptor expression (ICERE-1) in breast carcinomas
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DOI：
10.1046/j.1432-1327.1998.2520169.x
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发表时间：
1998-02-15
期刊：
影响因子：
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通讯作者：
Weigel, RJ
中科院分区：
文献类型：
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作者：
Thompson, DA;Weigel, RJ
使用差异显示比较两种雌激素受体（ER）阳性乳腺癌细胞系（MCF7和T-47D）和两种ER阴性乳腺癌细胞系（MDA-MB-231和HBL-100）的基因表达模式。鉴定出一个 377 bp 的片段，该片段在 ER 阴性细胞系中过度表达。该克隆的序列分析以及与 GenBank/EMBL 数据库的比较表明它与之前发表的任何基因都不匹配。该基因的表达模式与 ER 的表达呈负相关，被称为 ICERE-1（与雌激素受体表达负相关）。从 MDA-MB-231 cDNA 文库中分离出较长的 ICERE-1 克隆，序列分析表明该 2168 bp cDNA 包含编码 234 个氨基酸的蛋白质的 ORF，该蛋白质与任何先前描述的蛋白质序列几乎没有相似性。对一组乳腺癌细胞系的 Northern 印迹分析表明，大约 2.2 kb 的 ICERE-1 mRNA 在 ER 阴性乳腺癌细胞系 MDA-MB-231 和 HBL-100 以及 ER 阴性细胞系 HEC-1-B、HeLa 和 293 中大量表达。ICERE-1 的表达在 ER 阳性乳腺癌细胞系 MCF7、T-47D、 MDA-MB-361、ZR-75-1、BT-474 和 BT-20。使用逆转录/PCR检测29个原发性乳腺癌中ICERE-1的表达，其中15个经免疫组织化学鉴定为ER阳性，14个为ER阴性。 ICERE-1的表达水平显着降低（P
Differential display was used to compare patterns of gene expression in two estrogen receptor (ER)-positive breast carcinoma cell lines (MCF7 and T-47D) and two ER-negative breast carcinoma cell lines (MDA-MB-231 and HBL-100). A 377-bp fragment was identified that was overexpressed in the ER-negative cell lines. Sequence analysis of this clone and comparison with the GenBank/EMBL databases indicated that it did not match any genes published previously. The expression pattern of this gene was inversely correlated with the expression of ER and has been termed ICERE-1 (inversely correlated with estrogen receptor expression). A longer clone of ICERE-1 was isolated from a MDA-MB-231 cDNA library and sequence analysis indicated that this 2168-bp cDNA contained an ORF encoding a protein of 234 amino acids that bears little similarity with any previously described protein sequence. Northern blot analysis of a panel of breast cancer cell lines demonstrated that an ICERE-1 mRNA of approximately 2.2 kb was abundantly expressed in the ER-negative breast carcinoma cell lines, MDA-MB-231 and HBL-100, and the ER-negative cell lines, HEC-1-B, HeLa, and 293. Expression of ICERE-1 was absent or minimal in the ER-positive breast carcinoma cell lines MCF7, T-47D, MDA-MB-361, ZR-75-1, BT-474 and BT-20. Reverse transcription/PCR was used to examine ICERE-1 expression in 29 primary breast carcinomas, 15 of which had been designated as ER positive and 14 as ER negative by immunohistochemistry. The expression level of ICERE-1 was significantly lower (P