Mouse Ia antigens are receptors for lactate dehydrogenase virus
Mouse Ia antigens are receptors for lactate dehydrogenase virus
复制标题
小鼠 Ia 抗原是乳酸脱氢酶病毒的受体
作者：
T. Inada;C. Mims
乳酸脱氢酶病毒（LDV）感染小鼠可导致血浆乳酸脱氢酶水平升高、终身病毒血症以及细胞介导和体液免疫应答紊乱。病毒复制专门在一组有限的macrophages 1，2，但限制细胞易感性的基础是未知的。通过免疫荧光技术，我们发现感染的百分比与表达由主要组织相容性复合体（Ia）的I区编码的抗原的百分比相同。CBA株I-A+腹腔巨噬细胞的感染在同时用I-A和I-E单克隆抗体处理时被阻断，而单独用任一抗体处理则不能。用与小鼠I-A和I-E抗原同源的纯化的大鼠糖蛋白处理LDV时，病毒的感染性被灭活。这些结果表明LDV的受体是I-A和I-E抗原。选择性感染Ia阳性巨噬细胞可能对感染小鼠的免疫功能有重要影响。
Infection of mice with lactate dehydrogenase virus (LDV) leads to elevation of plasma lactate dehydrogenase, lifelong viraemia and perturbations of cell-mediated and humoral immune responses. The virus replicates exclusively in a restricted set of macrophages1,2, but the basis for restricted cell susceptibility is unknown. By immunofluorescence techniques we have found that the per cent infected was the same as the per cent expressing antigens encoded by the I region of the major histocompatibility complex (Ia). Infection of CBA strain I–A+ peritoneal macrophages was blocked when cells were treated simultaneously with monoclonal antibody to I–A and I–E, but not with either antibody separately. LDV infectivity was inactivated when virus was treated with purified rat glycoprotein homologous to mouse I–A and I–E antigens. These results indicate that the receptors for LDV are I–A and I–E antigens. Selective infection of Ia-positive macrophages may have an important effect on the immunological capability of infected mice.
乳酸脱氢酶升高病毒在巨噬细胞中的复制。
DOI：
10.1099/0022-1317-59-2-263
发表时间：
1982
期刊：
The Journal of general virology
影响因子：
--
作者：
Stueckemann,JA;Holth,M;Swart,WJ;Kowalchyk,K;Smith,MS;Wolstenholme,AJ;Cafruny,WA;Plagemann,PG
通讯作者：
Plagemann,PG
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Beller,DI;Unanue,ER
通讯作者：
Unanue,ER