Transcription of 4’-thioDNA templates to natural RNA in vitro and in mammalian cells.
Transcription of 4’-thioDNA templates to natural RNA in vitro and in mammalian cells.
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4’-thioDNA 模板在体外和哺乳动物细胞中转录为天然 RNA。
DOI：
10.1039/c4cc08862j
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Maruyama;H.; Furukawa;K.; Kamiya;H.; Minakawa;N.; Matsuda;A.
与DNA/RNA聚合酶相容的合成的化学修饰的核酸作为用于合成生物学研究的遗传材料具有巨大的潜力。本文报道了4′-thiDNA模板转录为天然RNA的综合分析。用2′-脱氧-4 ′-硫代糖苷修饰的DNA，即所谓的4′-硫代DNA，被发现在体外和哺乳动物细胞中作为RNA聚合酶转录的模板。
Synthetic chemically modified nucleic acids, which are compatible with DNA/RNA polymerases, have great potential as a genetic material for synthetic biological studies. A comprehensive analysis of the transcription of 4′-thioDNA templates to natural RNA is herein reported. The modifications of DNA with 2′-deoxy-4′-thionucleosides, so-called 4′-thioDNA, were found to act as templates for transcription with RNA polymerases both in vitro and in mammalian cells.