THE A-FORMS AND B-FORMS OF THE CHICKEN PROGESTERONE-RECEPTOR ARISE BY ALTERNATE INITIATION OF TRANSLATION OF A UNIQUE MESSENGER-RNA
THE A-FORMS AND B-FORMS OF THE CHICKEN PROGESTERONE-RECEPTOR ARISE BY ALTERNATE INITIATION OF TRANSLATION OF A UNIQUE MESSENGER-RNA
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DOI：
10.1016/0006-291x(87)90395-0
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发表时间：
1987-12-16
影响因子：
3.1
通讯作者：
OMALLEY, BW
中科院分区：
文献类型：
--
作者：
CONNEELY, OM;MAXWELL, BL;OMALLEY, BW
为了建立鸡孕酮受体的A和B蛋白的起源，我们在异源细胞中体内和体外网织红细胞裂解物中表达了其cDNA。A和B蛋白在异源受体阴性细胞和无细胞系统中均由单个cDNA表达。这两种蛋白质结合孕酮，并且在大小、免疫反应性和激素结合性质方面与鸡输卵管真实A和B蛋白质没有区别。 缺少受体B蛋白翻译信号的截短的mRNA能够通过在第二个内部起始位点启动翻译而产生受体A蛋白。我们从这些数据中得出结论，鸡孕酮受体A和B蛋白最有可能是由单个mRNA转录物的翻译交替起始而产生的。
In order to establish the origin of the A and B proteins of the chicken progesterone receptor we have expressed its cDNA in vivo in heterologous cells and in vitro in reticulocyte cell lysates. The A and B proteins were expressed from a single cDNA both in heterologous receptor negative cells and in a cell-free system. Both proteins bind progesterone and are indistinguishable from chick oviduct authentic A and B proteins in terms of size, immunoreactivity and hormone binding properties. Truncated mRNA''s which lack the receptor B protein translation signal are capable of generating the receptor A protein by initiation of translation at a second internal start site. We conclude from these data that the chicken progesterone receptor A and B proteins arise most likely by alternate initiation of translation from a single mRNA transcript.