CHEMICAL AND PHYSICAL BEHAVIOUR OF COLLAGEN FIBRE IN ALKALINE SOLUTIONS

CHEMICAL AND PHYSICAL BEHAVIOUR OF COLLAGEN FIBRE IN ALKALINE SOLUTIONS
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胶原纤维在碱性溶液中的化学和物理行为

DOI：
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发表时间：
2009
期刊：
JSLTC
影响因子：
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通讯作者：
CHEN MIN, LIAO LONGLI, LI ZHIQIANG
CHEN MIN, LIAO LONGLI, LI ZHIQIANG
中科院分区：
其他
文献类型：
--
作者：
CHENG HAIMING;CHEN MIN, LIAO LONGLI, LI ZHIQIANG

文献摘要

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研究了皮肤胶原纤维在碱性溶液中的化学和物理行为。经氢氧化钠和氢氧化钙溶液处理后，透射电镜未见胶原纤维特有的d期。纤维在碱溶液中溶胀，纤维直径增大。肿胀发生在纤维间和纤维内。胶原蛋白在碱性溶液中的水解产物呈扁平结构，说明水解产物在碱性溶液中发生了变性。当胶原纤维在碱溶液中长期处理时，水解胶原蛋白的等电点在4.5 ~ 5.0之间。碱溶液浓度越高，水解产物的等电点越低。在碱处理过程中，等电点向较低的pH值移动，处理48小时后等电点达到平衡，不再发生进一步变化。根据CD谱图，胶原蛋白在碱溶液中水解时，其三螺旋构象被破坏。SDS-PAGE电泳结果显示，碱溶液中胶原水解产物呈连续带状，未出现胶原特征带。胶原纤维在碱溶液中的水解不仅发生在分子间，而且发生在随机水解的胶原分子的肽键上。
The chemical and physical behaviour of skin collagen fibre was studied in alkali solutions. After treatment with sodium hydroxide and calcium hydroxide solutions, the characteristic D-period of collagen fibril could not be seen by TEM. The fibril diameter increased by swelling in alkali solutions. The swelling occurred both inter and intra fibrils. The hydrolysate of collagen in alkali solution was seen as a flat structure which illustrated that the hydrolysate was denatured in alkaline solutions. When collagen fibre was treated in alkali solutions for long periods, the isoelectric points of collagen hydrolysate were between 4.5 and 5.0. The higher the concentration of alkali solution, the lower the isoeletric point of the hydrolysate. During alkali treatments, the isoelectric point shifted to lower pH and, after 48 hour treatment, the isoelectric point reached equilibrium and did not change further. The triple helix conformation of collagen was destroyed when collagen was hydrolyzed in solutions of alkali according to the CD spectrum. SDS-PAGE electrophoresis results showed that hydrolysate of collagen in alkali solutions presented as a continuous band, no characteristic band of collagen appeared. The hydrolysis of collagen fibre in alkali solutions not only happened at inter-molecular points, but also happened at peptide bonds of collagen molecule which was randomly hydrolysed.