Anisotropy of collagen fibre alignment in bovine cartilage: comparison of polarised light microscopy and spatially resolved diffusion-tensor measurements
Anisotropy of collagen fibre alignment in bovine cartilage: comparison of polarised light microscopy and spatially resolved diffusion-tensor measurements
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DOI：
10.1016/j.joca.2007.09.015
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发表时间：
2008-06-01
影响因子：
7
通讯作者：
Momot, K. I.
中科院分区：
文献类型：
--
作者：
de Visser, S. K.;Bowden, J. C.;Momot, K. I.
目的：比较偏光显微镜（PLM）和弥散张量成像（DTI）对牛关节软骨胶原纤维排列角度的影响。方法：采用磁共振成像和PLM技术对5种不同动物的5个牛关节软骨样本进行研究。对每个样本获取T(2)加权、扩散张量（DT）和PLM图像，并比较PLM和DTI角度的平均深度分布，以及T(2)加权磁共振（MR）图像中观察到的带状模式。对DTI角和PLM角分布的统计性质进行了检验。结果：样品呈现出一系列的排列形态。在具有“常规”三区对准模式的样本中，观察到PLM和DTI对准区域与T(2)加权MR图像中的带状之间的相关性。每个样品的PLM和DTI对准角的深度轮廓形状在质量上相似。三个样品的DT和PLM对准角之间有很好的定量相关性。在光纤排列无序度较低的区域，扩散与PLM排列角之间的相关性最好。结论：本研究首次提供了软骨DTI与更成熟的PLM技术的定量比较。从PLM和DTI数据得出的对准角之间的相关性在广泛的对准形态中是明显的。结果支持使用DTI定量测量胶原纤维排列。微观尺度（类似于10 μ m）纤维取向角的色散似乎是理解PLM和DTI结果之间定量相关性程度的重要因素。(C) 2007国际骨关节炎研究学会。Elsevier Ltd.出版。版权所有。
Objective: To compare collagen fibre alignment angles obtained from polarised light microscopy (PLM) and diffusion-tensor imaging (DTI) in bovine articular cartilage.Methods: Five samples of bovine articular cartilage from five different animals were studied using magnetic resonance imaging and PLM techniques. T(2)-weighted, diffusion-tensor (DT), and PLM images were acquired for each sample and average depth profiles of the PLM and DTI angles, as well as the banding patterns observed in T(2)-weighted magnetic resonance (MR) images, were compared. Statistical properties of the distributions of the DTI and PLM angles were examined.Results: The samples exhibited a range of alignment morphologies. In the samples with the "conventional" three-zone alignment pattern, a correlation between the PLM and DTI alignment zones and the banding in T(2)-weighted MR images was observed. The shapes of the depth profiles of the PLM and DTI alignment angles were qualitatively similar for each sample. Three samples showed good quantitative correlation between the DT and PLM alignment angles. The correlation between the diffusion and PLM alignment angles was best in the regions of low degree of disorder of fibre alignment.Conclusions: This study provides the first quantitative comparison of DTI of cartilage with the more established PLM techniques. The correlation between alignment angles derived from PLM and DTI data was evident across a wide range of alignment morphologies. The results support the use of DTI for the quantitative measurement of collagen fibre alignment. The microscopic-scale (similar to 10 mu m) dispersion of fibre alignment angles appears to be an important factor for understanding the extent of quantitative correlation between PLM and DTI results. (C) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.