Impact of 3D Hierarchical Nanostructures on the Antibacterial Efficacy of a Bacteria-Triggered Self-Defensive Antibiotic Coating
Impact of 3D Hierarchical Nanostructures on the Antibacterial Efficacy of a Bacteria-Triggered Self-Defensive Antibiotic Coating
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DOI：
10.1021/acsami.5b05947
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发表时间：
2015-09-16
影响因子：
9.5
通讯作者：
Choi, Chang-Hwan
中科院分区：
文献类型：
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作者：
Hizal, Ferdi;Zhuk, Iryna;Choi, Chang-Hwan
钛经常用于种植手术，但经常与种植体表面细菌粘附和生长相关的感染有关。在这里，我们表明，钛的分层纳米结构以及随后用自卫性抗菌逐层（LbL）薄膜涂覆所得的形貌特征，可以实现分层纳米形貌和局部细菌触发的抗生素释放的协同作用，从而显着提高表面的抗菌效率。尽管钛基底的单独纳米结构并未显着影响金黄色葡萄球菌的粘附和生长，但用超薄单宁酸/庆大霉素 (TA/G) LbL 薄膜涂覆 3D 纳米柱基底可使表面附着的细菌数量减少 10 倍。这种效应归因于纳米结构涂层表面积的增大，可用于局部细菌触发的抗生素释放，以及细菌粘附力的降低，导致当细菌落在纳米柱尖端时，细菌抗生素防御机制的激活减弱。结果表明，3D 纳米结构与细菌触发的抗生素释放涂层的结合提供了一种独特的方法来显着增强生物材料植入物的抗菌功效。
Titanium is often applied in implant surgery, but frequently implicated in infections associated with bacterial adhesion and growth on the implant surface. Here, we show that hierarchical nanostructuring of titanium and the subsequent coating of resulting topographical features with a self-defensive, antibacterial layer-by-layer (LbL) film enables a synergistic action of hierarchical nanotopography and localized, bacteria-triggered antibiotic release to dramatically enhance the antibacterial efficiency of surfaces. Although sole nanostructuring of titanium substrates did not significantly affect adhesion and growth of Staphylococcus aureus, the coating of 3D-nanopillared substrates with an ultrathin tannic acid/gentamicin (TA/G) LbL film resulted in a 10-fold reduction of the number of surface-attached bacteria. This effect is attributed to the enlarged surface area of the nanostructured coating available for localized bacteria-triggered release of antibiotics, as well as to the lower bacterial adhesion forces resulting in subsided activation of bacterial antibiotic-defense mechanisms when bacteria land on nanopillar tips. The result shows that a combination of 3D nanostructuring with a bacteria-triggered antibiotic-releasing coating presents a unique way to dramatically enhance antibacterial efficacy of biomaterial implants.