INDUCTION OF A CYTOGENETIC ADAPTIVE RESPONSE IN GERM-CELLS OF IRRADIATED MICE WITH VERY LOW-DOSE RATE OF CHRONIC GAMMA-IRRADIATION AND ITS BIOLOGICAL INFLUENCE ON RADIATION-INDUCED DNA OR CHROMOSOMAL DAMAGE AND CELL-KILLING IN THEIR MALE OFFSPRING
INDUCTION OF A CYTOGENETIC ADAPTIVE RESPONSE IN GERM-CELLS OF IRRADIATED MICE WITH VERY LOW-DOSE RATE OF CHRONIC GAMMA-IRRADIATION AND ITS BIOLOGICAL INFLUENCE ON RADIATION-INDUCED DNA OR CHROMOSOMAL DAMAGE AND CELL-KILLING IN THEIR MALE OFFSPRING
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DOI：
10.1093/mutage/10.2.95
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发表时间：
1995-03-01
期刊：
影响因子：
2.7
通讯作者：
WANG, P
中科院分区：
文献类型：
--
作者：
CAI, L;WANG, P
在早期的研究中，我们发现无论是单次照射还是多次照射，小鼠生殖细胞都能产生显著的细胞遗传学适应性反应。本文采用极低剂量率(20MU·Gy/min)的慢性Co-60γ射线照射小鼠40天，40天后，再进行大剂量X射线照射，24小时后对其精母细胞进行细胞遗传学分析。对小鼠脾细胞、骨髓细胞和小鼠精母细胞进行了辐射损伤分析。结果表明：(1)剂量率为20mU·GY/min的累积伽马辐射(1.10GY)对小鼠生殖细胞(精原干细胞)有明显的细胞遗传学适应性反应，(2)子代骨髓细胞和精母细胞对1.5GyX射线诱发的染色体畸变的敏感性不受低剂量照射到父本生殖细胞的影响，(3)子代脾细胞的DNA修复能力(UV诱导的非程序DNA合成，UDS)没有改变；(Iv)子代脾细胞对辐射诱导的细胞杀伤的敏感性也没有改变，这些结果表明，向雄性父母提供低剂量辐射，并显著诱导其生殖细胞的细胞遗传学适应性反应，可能不会对受辐射的雄性小鼠的后代造成任何损害。
In earlier studies we have shown that either a single exposure or multiple exposures to a low dose of X-rays (0.05 Gy) induced a significant cytogenetic adaptive response in mouse germ cells, In this paper, a very low-dose rate (20 mu Gy/min) of chronic Co-60 gamma-irradiation was used to pre-irradiate mice for 40 days, Then, another 40 days later, these mice were treated with a subsequent large dose of X-irradiation, followed 24 h later by cytogenetic analysis of their spermatocytes. Analysis for radiation-induced DNA and chromosomal damage was also carried out in splenocytes, bone marrow cells and spermatocytes of the offspring of mice adapted by the low-dose rate of chronic gamma-irradiation. Results demonstrated that (i) cumulative gamma-irradiation (1.10 Gy) at the dose rate 20 mu Gy/min induced a marked cytogenetic adaptive response in the mouse germ cells (stem spermatogonia); (ii) the sensitivity of offspring's bone marrow cells and spermatocytes to 1.5 Gy X-ray-induced chromosome aberrations was not influenced by the low-dose radiation delivered to paternal germ cells; (iii) either constitutive or post-irradiation DNA repair capacity (UV-induced unscheduled DNA synthesis, UDS) was not modified in the offspring's splenocytes; (iv) the sensitivity of the offspring's splenocytes to radiation-induced cell killing was also not altered, These results suggest that low-dose radiation delivered to the male parents with a significant induction of cytogenetic adaptive response in their germ cell does not likely cause any risk of damaging effects to the offspring of those irradiated male mice.