A Method for Growing Tissue-Isolated Human Tumor Xenografts in Nude Rats for Melatonin/Cancer Studies.
A Method for Growing Tissue-Isolated Human Tumor Xenografts in Nude Rats for Melatonin/Cancer Studies.
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一种在裸鼠中培养组织分离的人类肿瘤异种移植物用于褪黑激素/癌症研究的方法。
DOI：
10.1007/978-1-0716-2593-4_47
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Blask,DavidE
中科院分区：
文献类型：
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作者：
Dauchy,RobertT;Hill,StevenM;Blask,DavidE
组织分离肿瘤模型允许研究褪黑激素对实验室大鼠体内人类肿瘤生长和代谢的影响。在这里，我们描述了一种独特的手术技术，用于在由裸大鼠上腹部动脉和静脉组成的血管柄上植入和生长人肿瘤异种移植物，该血管柄为组织分离的肿瘤提供来自单一来源的连续血液供应，同时确保不存在外部血管连接。利用这种独特的模型，可以植入和生长多种人类肿瘤类型，该模型可以提供来自所检查的单个肿瘤的大量科学数据。
The tissue-isolated tumor model permits the investigation of melatonin’s influence on human tumor growth and metabolism in laboratory rats in vivo. Here we describe a unique surgical technique for implanting and growing human tumor xenografts on a vascular stalk composed of the nude rat epigastric artery and vein that provides a continuous blood supply from a single source to the tissue-isolated tumor while insuring the absence of extraneous vascular connections. A variety of human tumor types may be implanted and grown utilizing this unique model that may provide a plethora of scientific data from a single tumor examined.