Effect of bovine pericardial extracellular matrix scaffold niche on seeded human mesenchymal stem cell function.
Effect of bovine pericardial extracellular matrix scaffold niche on seeded human mesenchymal stem cell function.
复制标题
牛心包外基质基质支架小裂对种子间充质干细胞功能的影响。
DOI：
10.1038/srep37089
复制
发表时间：
2016-11-15
影响因子：
4.6
通讯作者：
Griffiths LG
中科院分区：
文献类型：
--
作者：
Liu ZZ;Wong ML;Griffiths LG
Numerous studies have focused on generation of unfixed bovine pericardium (BP) extracellular matrix (ECM) for clinical application. However, the extent to which maintenance of native ECM niche is capable of directing behavior of repopulating cells remains relatively unexplored. By exploiting the sidedness of BP scaffolds (i.e., serous or fibrous surface), this study aims to determine the effect of ECM niche preservation on cellular repopulation using different scaffold generation methods. BP underwent either sodium dodecyl sulfate (SDS) decellularization or stepwise, solubilization-based antigen removal using amidosulfobetaine-14 (ASB-14). SDS scaffolds were toxic to repopulating human mesenchymal stem cells (hMSC). Scanning electron microscopy revealed distinct surface ultrastructure of ASB-14 scaffolds based on native BP sidedness. Basement membrane structures on the serous side stimulated hMSC cell monolayer formation, whereas fibrous side facilitated cell penetration into scaffold. Additionally, serous side seeding significantly increased hMSC adhesion and proliferation rate compared to the fibrous side. Furthermore, scaffold ECM niche stimulated sidedness dependent differential hMSC human leukocyte antigen expression, angiogenic and inflammatory cytokine secretion. This work demonstrates that ECM scaffold preparation method and preservation of BP side-based niches critically affects in vitro cell growth patterns and behavior, which has implications for use of such ECM biomaterials in clinical practice.
登录
查看更多内容
影响因子：
4.6
作者：
IONESCU, MI;SMITH, DR;TANDON, AP
通讯作者：
TANDON, AP
影响因子：
2.7
作者：
Calin, Manuela;Stan, Daniela;Simion, Viorel
通讯作者：
Simion, Viorel
影响因子：
--
作者：
Gratzer, Paul F.;Harrison, Robert D.;Woods, Terence
通讯作者：
Woods, Terence
影响因子：
4.9
作者：
Harrison, RD;Gratzer, PF
通讯作者：
Gratzer, PF
影响因子：
2.2
作者：
Barakat, Omar;Abbasi, Shahrzad;Gauthier, Polly K.
通讯作者：
Gauthier, Polly K.