COOPERATION BETWEEN CORE PROMOTER ELEMENTS INFLUENCES TRANSCRIPTIONAL ACTIVITY IN-VIVO
COOPERATION BETWEEN CORE PROMOTER ELEMENTS INFLUENCES TRANSCRIPTIONAL ACTIVITY IN-VIVO
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DOI：
10.1073/pnas.92.6.1955
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发表时间：
1995-03-14
影响因子：
11.1
通讯作者：
MANLEY, JL
中科院分区：
文献类型：
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作者：
COLGAN, J;MANLEY, JL
RNA聚合酶II的核心启动子通常含有两个共有序列元件中的一个（或两个），TATA盒和/或起始子（Inr）。使用由原型TATA和/或Inr元件组成的测试启动子，连同序列特异性激活剂的结合位点，我们分析了TATA和Inr元件在体内的功能。在没有激活剂的情况下，TATA元件比Inr显着更活跃，并且元件的组合仅比仅TATA启动子稍微更有效。在任何几个共表达的激活蛋白的存在下，TATA元件再次是最活跃的，但这里除了允许的INR活性显着增加，表明两个元素之间的合作相互作用。一个有趣的例外是观察到的激活剂Sp1，这是更有效的Inr-唯一的启动子，并添加一个TATA框没有增强活性。最后，在所有情况下，发现TATA加Inr启动子部分或完全抵抗先前显示干扰仅TATA启动子表达的转录因子TFIIB突变体的显性负效应。这一结果加强了TATA和Inr元件在体内可以协同作用的结论。
Core promoters for RNA polymerase II frequently contain either (or both) of two consensus sequence elements, a TATA box and/or an initiator (Inr). Using test promoters consisting of prototypical TATA and/or Inr elements, together with binding sites for sequence-specific activators, we have analyzed the function of TATA and Inr elements in vivo. In the absence of activators, the TATA element was significantly more active than the Inr, and the combination of elements was only slightly more effective than the TATA-only promoter. In the presence of any of several coexpressed activator proteins, the TATA element was again most active, but here addition of the Inr allowed significant increases in activity, indicating a cooperative interaction between the two elements. An interesting exception was observed with the activator Sp1, which was more effective with the Inr-only promoter, and addition of a TATA box did not enhance activity. Finally, in all cases the TATA plus Inr promoters were found to be partially or completely resistant to the dominant negative effects of a transcription factor TFIIB mutant previously shown to interfere with expression from TATA-only promoters. This result strengthens the conclusion that TATA and Inr elements can cooperate in vivo.