INDUCTION OF LIVER CYTOCHROME-P450 2B1 BY BETA-IONONE IN SPRAGUE-DAWLEY RATS
INDUCTION OF LIVER CYTOCHROME-P450 2B1 BY BETA-IONONE IN SPRAGUE-DAWLEY RATS
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DOI：
10.1006/bbrc.1995.2610
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发表时间：
1995-11-02
影响因子：
3.1
通讯作者：
ROH, JK
中科院分区：
文献类型：
--
作者：
JEONG, TC;KIM, HJ;ROH, JK
研究了β -离子酮对雄性和雌性大鼠肝细胞色素P450 2B1的诱导作用。在动物牺牲前72和48小时皮下注射β -离子酮，不仅显著诱导了己氧基间苯二酚o脱烷基酶的肝微粒体活性，而且细胞色素P450 2B1蛋白水平明显升高。β -离子酮对雄性大鼠细胞色素P450 2B1的诱导作用明显大于雌性大鼠。凹槽印迹分析显示，雄性大鼠在β -离子酮治疗后6小时，雌性大鼠在治疗后12小时mRNA水平升高。综上所述，本研究结果首次表明β -离子酮对细胞色素P450 2B1的诱导可能受mrna积累的调节。(C) 1995学术出版社，Inc。
Induction of liver cytochrome P450 2B1 by beta-ionone was investigated in male and female Sprague Dawley rats. Administration of beta-ionone subcutaneously 72 and 48 hr before sacrificing the animals not only significantly induced the liver microsomal activity of pentoxyresorufin O-dealkylase, but also clearly increased in the level of cytochrome P450 2B1 protein. The induction of cytochrome P450 2B1 by beta-ionone was much greater in male rats than in female rats. A slot blot analysis showed that the mRNA level was increased from 6 hr after treatment with beta-ionone in male rats and from 12 hr after treatment in female rats. Taken together, the present results indicate for the first time that the induction of cytochrome P450 2B1 by beta-ionone might be regulated by the accumulation of mRNAs. (C) 1995 Academic Press, Inc.