Lagrangian 3D tracking of fluorescent microscopic objects in motion.
Lagrangian 3D tracking of fluorescent microscopic objects in motion.
复制标题
运动中荧光微观物体的拉格朗日 3D 跟踪。
DOI：
10.1063/1.4982820
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
É. Clément
中科院分区：
文献类型：
--
作者：
T. Darnige;Nuris Figueroa;P. Bohec;A. Lindner;É. Clément
我们描述了一种跟踪装置的发展，安装在一个外延荧光倒置显微镜，适合于获得时间分辨率的三维拉格朗日轨迹的荧光被动或主动微流体装置。该系统基于实时图像处理，确定x、y机械台的位移，使所选物体保持在观测帧内的固定位置。z位移是基于荧光物体的重新聚焦，确定压电移动器的位移，使运动物体保持聚焦。物体相对于微流控装置的轨迹坐标以及物体的图像以十分之一赫兹的频率获得。该装置特别适合于在有或没有流动的微流体装置中获得活动微生物的轨迹。
We describe the development of a tracking device, mounted on an epi-fluorescent inverted microscope, suited to obtain time resolved 3D Lagrangian tracks of fluorescent passive or active micro-objects in microfluidic devices. The system is based on real-time image processing, determining the displacement of a x, y mechanical stage to keep the chosen object at a fixed position in the observation frame. The z displacement is based on the refocusing of the fluorescent object determining the displacement of a piezo mover keeping the moving object in focus. Track coordinates of the object with respect to the microfluidic device as well as images of the object are obtained at a frequency of several tenths of Hertz. This device is particularly well adapted to obtain trajectories of motile micro-organisms in microfluidic devices with or without flow.
影响因子：
3.4
作者：
L. Turner;L. Ping;Marianna Neubauer;H. Berg
通讯作者：
L. Turner;L. Ping;Marianna Neubauer;H. Berg
影响因子：
16.6
作者：
Jikeli JF;Alvarez L;Friedrich BM;Wilson LG;Pascal R;Colin R;Pichlo M;Rennhack A;Brenker C;Kaupp UB
通讯作者：
Kaupp UB