DNA-mediated inhibition of peroxidase-like activities on platinum nanoparticles for simple and rapid colorimetric detection of nucleic acids
DNA-mediated inhibition of peroxidase-like activities on platinum nanoparticles for simple and rapid colorimetric detection of nucleic acids
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DNA 介导的铂纳米粒子过氧化物酶样活性抑制，用于简单快速的核酸比色检测
DOI：
10.1016/j.bios.2017.02.025
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发表时间：
2017-08-15
影响因子：
12.6
通讯作者：
Kong, Jilie
中科院分区：
文献类型：
--
作者：
Chen, Weiwei;Fang, Xueen;Kong, Jilie
在本研究中，我们发现，非共价DNA-Pt杂化纳米粒子（PtNPs）的过氧化物酶样活性，可以明显阻断，当使用DNA作为模板原位合成铂纳米粒子（PtNPs）。此外，这种自组装合成过程非常方便和快速（几分钟内），并且DNA介导的抑制作用也非常有效。首先，通过纸基分析装置（PAD），我们发现DNA-Pt杂化纳米粒子的催化活性与DNA浓度在0.0075 - 0.25 μ M范围内呈线性响应。利用磁珠分离系统和靶DNA诱导的杂交链反应（HCR），实现了特异性靶DNA分析，检测限为0.228 nM，并证明了该方法在区分靶DNA和其它干扰物方面的有效性。据我们所知，这是第一个使用DNA和PtNPs之间的纳米组装体用于核酸比色检测的报告，这是基于DNA介导的铂纳米颗粒催化活性的抑制。这一结果对于理解DNA与金属纳米粒子之间的相互作用以及开发其他方便有效的分析策略具有重要意义。
In this research, we found that the peroxidase-like activities of noncovalent DNA-Pt hybrid nanoparticles could be obviously blocked, when Pt nanoparticles (PtNPs) were synthesized in situ using DNA as a template. Moreover, this self-assembled synthetic process was very convenient and rapid (within few mintues), and the inhibition mediated by DNA was also very effective. First, by the paper-based analytical device (PAD) we found the catalytic activities of DNA-Pt hybrid nanoparticles exhibited a linear response to the concentration of DNA in the range from 0.0075 to 0.25 mu M. Then, with the magnetic bead isolated system and target DNA-induced hybridization chain reaction (HCR), we realized the specific target DNA analysis with a low detection of 0.228 nM, and demonstrated its effectivity in distinguishing the target DNA from other interferences. To our knowledge, this is the first report that used the nanoassembly between DNA and PtNPs for colorimetric detection of nucleic acids, which was based on DNA-mediated inhibition of catalytic activities of platinum nanoparticles. The results may be useful for understanding the interactions between DNA and metal nanoparticles, and for development of other convenient and effective analytical strategies.