Integrated Transcriptomic, Proteomic, and Metabolomics Analysis Reveals Peel Ripening of Harvested Banana under Natural Condition
Integrated Transcriptomic, Proteomic, and Metabolomics Analysis Reveals Peel Ripening of Harvested Banana under Natural Condition
复制标题
综合转录组学、蛋白质组学和代谢组学分析揭示自然条件下收获香蕉的果皮成熟
DOI：
10.3390/biom9050167
复制
发表时间：
2019-05-01
期刊：
影响因子：
5.5
通讯作者：
Duan, Xuewu
中科院分区：
文献类型：
--
作者：
Yun, Ze;Li, Taotao;Duan, Xuewu
香蕉成熟是一个复杂的生理生化过程，果肉和果皮的成熟调控存在差异。然而，有关果皮成熟的分子机制仍然没有得到很好的理解。在这项研究中，我们进行了组合的转录组学，蛋白质组学和代谢组学分析香蕉果实成熟过程中的果皮。结果表明，香蕉成熟过程中果皮中有5784个基因、94个蛋白质和133种代谢产物的差异表达或积累。这些基因和蛋白质与成熟相关的过程有关，包括转录调控、激素信号传导、细胞壁修饰、香气合成、蛋白质修饰和能量代谢。差异表达的转录因子主要是乙烯反应因子（ERF）和碱性螺旋-环-螺旋（bHLH）家族成员。此外，大量生长素信号相关基因表达上调，外源3-吲哚乙酸（IAA）处理促进香蕉果实成熟，并上调了许多成熟相关基因的表达，表明生长素参与了香蕉果皮成熟的调控。此外，木葡聚糖内切转葡糖基酶/水解酶（XTH）家族成员在果皮软化中起重要作用。热激蛋白（Hsps）介导的蛋白质修饰和泛素-蛋白体系统介导的蛋白质降解都参与了果皮的成熟。香蕉果实成熟过程中果皮的能量代谢可能以无氧呼吸为主。总之，我们的研究突出了更好地了解香蕉果皮成熟的机制，并为进一步解剖特定基因的功能提供了新的线索。
Harvested banana ripening is a complex physiological and biochemical process, and there are existing differences in the regulation of ripening between the pulp and peel. However, the underlying molecular mechanisms governing peel ripening are still not well understood. In this study, we performed a combination of transcriptomic, proteomic, and metabolomics analysis on peel during banana fruit ripening. It was found that 5784 genes, 94 proteins, and 133 metabolites were differentially expressed or accumulated in peel during banana ripening. Those genes and proteins were linked to ripening-related processes, including transcriptional regulation, hormone signaling, cell wall modification, aroma synthesis, protein modification, and energy metabolism. The differentially expressed transcriptional factors were mainly ethylene response factor (ERF) and basic helix-loop-helix (bHLH) family members. Moreover, a great number of auxin signaling-related genes were up-regulated, and exogenous 3-indoleacetic acid (IAA) treatment accelerated banana fruit ripening and up-regulated the expression of many ripening-related genes, suggesting that auxin participates in the regulation of banana peel ripening. In addition, xyloglucan endotransglucosylase/hydrolase (XTH) family members play an important role in peel softening. Both heat shock proteins (Hsps) mediated-protein modification, and ubiqutin-protesome system-mediated protein degradation was involved in peel ripening. Furthermore, anaerobic respiration might predominate in energy metabolism in peel during banana ripening. Taken together, our study highlights a better understanding of the mechanism underlying banana peel ripening and provides a new clue for further dissection of specific gene functions.