IRON ACQUISITION AND HEMOLYSIN PRODUCTION BY CAMPYLOBACTER-JEJUNI
IRON ACQUISITION AND HEMOLYSIN PRODUCTION BY CAMPYLOBACTER-JEJUNI
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DOI：
10.1128/iai.60.9.3872-3877.1992
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发表时间：
1992-09-01
影响因子：
3.1
通讯作者：
JUSUF, SSD
中科院分区：
文献类型：
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作者：
PICKETT, CL;AUFFENBERG, T;JUSUF, SSD
测试了空肠弯曲杆菌菌株从人类存在的各种铁源获取铁的能力。血红素、血红蛋白、血红素-血结合蛋白和血红蛋白-触珠蛋白刺激空肠弯曲菌菌株在低铁培养基中的生长。转铁蛋白、乳铁蛋白和铁蛋白无法为测试菌株提供铁。天然可转化的空肠弯曲菌菌株81-176的衍生物是基于它们不能使用氯高铁血红素作为铁源而被分离的。这些突变体也无法使用血红蛋白、血红素-血结合蛋白或血红蛋白-触珠蛋白作为铁源。一些突变体缺乏 71,000 Da 铁调节外膜蛋白，而其他突变体似乎保留了所有外膜蛋白。利用生长曲线和利用自然转化的重组实验来进一步表征突变体。一些空肠弯曲菌菌株显示出溶血活性，但它似乎不受铁调节。
Campylobacter jejuni strains were tested for their ability to acquire iron from various iron sources present in humans. Hemin, hemoglobin, hemin-hemopexin, and hemoglobin-haptoglobin stimulated the growth of C. jejuni strains in low-iron medium. Transferrin, lactoferrin, and ferritin were unable to provide iron to the strains tested. Derivatives of the naturally transformable C. jejuni strain 81-176 were isolated on the basis of their inability to use hemin as an iron source. These mutants were also unable to use hemoglobin, hemin-hemopexin, or hemoglobin-haptoglobin as iron sources. Some mutants lacked a 71,000-Da iron-regulated outer membrane protein, while others appeared to retain all of their outer membrane proteins. Growth curves and a recombination experiment that exploited natural transformation were used to further characterize the mutants. A hemolytic activity was shown to be produced by several C. jejuni strains, but it did not appear to be iron regulated.