IDENTIFICATION OF TOMATO BUSHY STUNT VIRUS HOST-SPECIFIC SYMPTOM DETERMINANTS BY EXPRESSION OF INDIVIDUAL GENES FROM A POTATO-VIRUS-X VECTOR
IDENTIFICATION OF TOMATO BUSHY STUNT VIRUS HOST-SPECIFIC SYMPTOM DETERMINANTS BY EXPRESSION OF INDIVIDUAL GENES FROM A POTATO-VIRUS-X VECTOR
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DOI：
10.1105/tpc.7.8.1157
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发表时间：
1995-08-01
期刊：
影响因子：
11.6
通讯作者：
JACKSON, AO
中科院分区：
文献类型：
--
作者：
SCHOLTHOF, HB;SCHOLTHOF, KBG;JACKSON, AO
在这项研究中，我们分析了两个嵌套基因（p19和p22）的番茄丛矮病毒（TBSV）的疾病症状的影响，在系统感染的植物和当地的病变寄主。通过用野生型TBSV的感染性克隆、用p19/p22突变衍生物的生物测定以及通过从异源马铃薯X病毒（PVX）载体表达单个TBSV基因来确定单个基因的贡献。结果表明，TBSV基因在PVX载体中得到了高效表达。这些蛋白质的亚细胞定位以及PVX表达的p22反式补体TBSV细胞间运动缺陷突变体的能力表明外源表达的蛋白质具有功能活性。用TBSV突变体和PVX衍生物进行的接种研究表明，p19在系统感染本氏烟草（Nicotiana benthamiana）和烟草黑胫病菌（N.克莱维兰地。此外，p19还可引起N. tabacum;而在N. nanososa和N. edwardsonii，局部损伤反应被p22激活。这些结果表明，猪和p22蛋白的TBSV是重要的症状决定因素，密切相关的植物物种可能含有不同的抗性基因，选择性地响应个别TBSV蛋白。
In this study, we analyzed the influence of two nested genes (p19 and p22) of tomato bushy stunt virus (TBSV) on disease symptoms in systemically infected plants and in local lesion hosts. The contribution of individual genes was determined by bioassays with an infectious clone of wild-type TBSV, with p19/p22 mutant derivatives, and by expression of individual TBSV genes from a heterologous potato virus X (PVX) vector. Our results showed that TBSV genes could be expressed at high levels from the PVX vector. The subcellular localization of these proteins as well as the ability of PVX-expressed p22 to trans complement TBSV cell-to-cell movement defective mutants indicate that the exogenously expressed proteins are functionally active. Inoculation studies with TBSV mutants and the PVX derivatives demonstrated that p19 induced a generalized necrosis upon systemic infection of Nicotiana benthamiana and N. clevelandii. In addition, p19 elicited the formation of local necrotic lesions in N. tabacum; however, in N. glutinosa and N. edwardsonii, the local lesion response was activated by p22. These results show that the pig and p22 proteins of TBSV are important symptom determinants and that closely related plant species may contain different resistance genes that selectively respond to individual TBSV proteins.