The BED finger domain protein MIG-39 halts migration of distal tip cells in Caenorhabditis elegans.
The BED finger domain protein MIG-39 halts migration of distal tip cells in Caenorhabditis elegans.
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BED 指结构域蛋白 MIG-39 阻止秀丽隐杆线虫远端细胞的迁移。
DOI：
10.1016/j.ydbio.2014.10.008
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Mitani S and Nishiwaki K
中科院分区：
文献类型：
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作者：
Kikuchi T;Shibata Y;Kim H.-S;Kubota Y;Yoshina S;Mitani S and Nishiwaki K
器官通常由上皮管的延伸和分支形成。上皮管延伸的适当终止对于产生适当大小和形态的器官是重要的。然而，上皮管终止其延伸的机制大多是未知的。在这里，我们发现bed -指结构域蛋白MIG-39可以阻止秀丽隐杆线虫上皮管的延伸。性腺领导细胞，被称为远端尖端细胞（dtc），在幼虫发育过程中以u形模式迁移，并在青年期停止迁移，产生具有前后u形臂的性腺。然而，Inmig-39突变体的dtc超过了它们正常的停止位置。米格-39促进了DTC的减速，使得DTC停止迁移的时机和定位合适。在3个Rac GTPase基因中，突变-10和突变-2增强了mig-39突变体的前dtc过调，而抑制了后dtc过调。另一方面，突变mig-2抑制了mig-39的前后DTC缺陷。遗传分析表明，MIG-39与Rac GTPases并行作用，阻止DTC迁移。我们提出了一个模型，其中前部和后部的DTC在停止DTC迁移时以相反的方式响应Rac活动水平。
Organs are often formed by the extension and branching of epithelial tubes. An appropriate termination of epithelial tube extension is important for generating organs of the proper size and morphology. However, the mechanism by which epithelial tubes terminate their extension is mostly unknown. Here we show that the BED-finger domain protein MIG-39 acts to stop epithelial tube extension inCaenorhabditis elegans. The gonadal leader cells, called distal tip cells (DTCs), migrate in a U-shaped pattern during larval development and stop migrating at the young adult stage, generating a gonad with anterior and posterior U-shaped arms. Inmig-39 mutants, however, DTCs overshot their normal stopping position. MIG-39 promoted the deceleration of DTCs, leading to the proper timing and positioning of the cessation of DTC migration. Among three Rac GTPase genes, mutations inced-10andrac-2enhanced the overshoot of anterior DTCs, while they suppressed that of posterior DTCs ofmig-39mutants. On the other hand, the mutation inmig-2suppressed both the anterior and posterior DTC defects ofmig-39. Genetic analyses suggested that MIG-39 acts in parallel with Rac GTPases in stopping DTC migration. We propose a model in which the anterior and posterior DTCs respond in an opposite manner to the levels of Rac activities in the cessation of DTC migration.