Characterization of mitochondrial genome of Chinese wild mulberry silkworm, Bomyx mandarina (Lepidoptera: Bombycidae)
Characterization of mitochondrial genome of Chinese wild mulberry silkworm, Bomyx mandarina (Lepidoptera: Bombycidae)
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DOI：
10.1007/s11427-008-0097-6
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发表时间：
2008-08-01
期刊：
影响因子：
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通讯作者：
Xiang ZhongHuai
中科院分区：
文献类型：
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作者：
Pan MinHui;Yu QuanYou;Xiang ZhongHuai
测定了中国家蚕（ChBm）的完整线粒体基因组。环状基因组长15682 bp，除了两个额外的tRNA样结构：tRNA(Ser(TGA))样和tRN(AIle(TAT))样之外，包含与家蚕(B. mori)和日本家蚕(JaBm)相同的典型基因互补、顺序和排列。所有蛋白质编码序列均以典型的 ATN 密码子起始，COI 基因除外，该基因具有 4 bp TTAG 推定起始密码子。 13 个蛋白质编码基因 (PCG) 中有 11 个具有完整终止密码子（均为 TAA），但其余两个基因以不完整密码子终止。所有 tRNA 均具有线粒体 tRNA 的典型三叶草结构，但 tRNA(Ser(TGA)) 样除外，具有四茎环结构。 ChBm 富含 A+T 的区域长度为 484 bp，比 JaBm (747 bp) 和家蚕 (494-499 bp) 的长度短。 B. mori、ChBm、JaBm 和 Antheraea pernyi (Anpe) 之间的系统发育分析表明，B. mori 与 ChBm 的亲缘关系比 JaBm 更近。 B. mori-ChBm 和 B. mori-JaBm 的最早分歧时间估计分别约为 1.08 +/- 0.18-1.41 +/- 0.24 和 1.53 +/- 0.20-2.01 +/- 0.26 Mya。 ChBm 和 JaBm 的差异约为 1.11 +/- 0.16-1.45 +/- 0.21 Mya。
The complete mitochondrial genome of Chinese Bombyx mandarina (ChBm) was determined. The circular genome is 15682 bp long, and contains a typical gene complement, order, and arrangement identical to that of Bombyx mori (B. mori) and Japanese Bombyx mandarina (JaBm) except for two additional tRNA-like structures: tRNA(Ser(TGA))-like and tRN(AIle(TAT))-like. All protein-coding sequences are initiated with a typical ATN codon except for the COI gene, which has a 4-bp TTAG putative initiator codon. Eleven of 13 protein-coding genes (PCGs) have a complete termination codon (all TAA), but the remaining two genes terminate with incomplete codons. All tRNAs have the typical clover-leaf structures of mitochondrial tRNAs, with the exception of tRNA(Ser(TGA))-like, with a four stem-and-loop structure. The length of the A+T-rich region of ChBm is 484 bp, shorter than those of JaBm (747 bp) and B. mori (494-499 bp). Phylogenetic analysis among B. mori, ChBm, JaBm, and Antheraea pernyi (Anpe) showed that B. mori is more closely related to ChBm than JaBm. The earliest divergence time estimate for B. mori-ChBm and B. mori-JaBm is about 1.08 +/- 0.18-1.41 +/- 0.24 and 1.53 +/- 0.20-2.01 +/- 0.26 Mya, respectively. ChBm and JaBm diverged around 1.11 +/- 0.16-1.45 +/- 0.21 Mya.