Preparation of Antioxidant Protein Hydrolysates from Pleurotus geesteranus and Their Protective Effects on H(2)O(2) Oxidative Damaged PC12 Cells.
Preparation of Antioxidant Protein Hydrolysates from Pleurotus geesteranus and Their Protective Effects on H(2)O(2) Oxidative Damaged PC12 Cells.
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DOI：
10.3390/molecules25225408
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发表时间：
2020-11-19
期刊：
影响因子：
--
通讯作者：
Ding Y
中科院分区：
文献类型：
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作者：
Liao X;Zhu Z;Wu S;Chen M;Huang R;Wang J;Wu Q;Ding Y
Pleoprogeesteranus是一种有前途的生物活性化合物来源。然而，目前对秀珍菇蛋白水解物（PGPHs）抗氧化性的研究还很有限。本研究分别用木瓜蛋白酶、碱性蛋白酶、风味蛋白酶、胃蛋白酶和胰酶制备PGPH。采用不同的化学方法和H_2O_2损伤的PC12细胞，研究了PGPH的抗氧化特性和抗氧化应激的细胞保护作用。结果表明，PGPHs具有良好的上级抗氧化活性。其中碱性蛋白酶水解产物对DPPH自由基的清除率最高（91.62%），对ABTS自由基的清除率最高（90.53%），对铁离子的还原性抗氧化能力最强，对金属离子的螯合能力最强（82.16%）。氨基酸组成分析表明，该水解液富含疏水性、带负电荷和芳香族氨基酸，有助于其上级抗氧化性能。此外，碱性蛋白酶水解物显示细胞保护作用H2O2诱导的氧化应激在PC12细胞通过减少细胞内活性氧（ROS）的积累，刺激抗氧化酶的活性。综上所述，碱性蛋白酶水解的P.geesteranus蛋白可以用作具有抗氧化特性和对ROS介导的氧化应激的保护作用的有益成分。
Pleurotus geesteranus is a promising source of bioactive compounds. However, knowledge of the antioxidant behaviors of P. geesteranus protein hydrolysates (PGPHs) is limited. In this study, PGPHs were prepared with papain, alcalase, flavourzyme, pepsin, and pancreatin, respectively. The antioxidant properties and cytoprotective effects against oxidative stress of PGPHs were investigated using different chemical assays and H2O2 damaged PC12 cells, respectively. The results showed that PGPHs exhibited superior antioxidant activity. Especially, hydrolysate generated by alcalase displayed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (91.62%), 2,2-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) radical scavenging activity (90.53%), ferric reducing antioxidant power, and metal ion-chelating activity (82.16%). Analysis of amino acid composition revealed that this hydrolysate was rich in hydrophobic, negatively charged, and aromatic amino acids, contributing to its superior antioxidant properties. Additionally, alcalase hydrolysate showed cytoprotective effects on H2O2-induced oxidative stress in PC12 cells via diminishing intracellular reactive oxygen species (ROS) accumulation by stimulating antioxidant enzyme activities. Taken together, alcalase hydrolysate of P. geesteranus protein can be used as beneficial ingredients with antioxidant properties and protective effects against ROS-mediated oxidative stress.