Toxicological analysis of chlorhexidine in human serum using HPLC on a polymer-coated ODS column
Toxicological analysis of chlorhexidine in human serum using HPLC on a polymer-coated ODS column
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DOI：
10.1093/jat/26.2.119
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发表时间：
2002-03-01
影响因子：
2.5
通讯作者：
Inoue, N
中科院分区：
文献类型：
--
作者：
Kudo, K;Ikeda, N;Inoue, N
建立了人血清中氯己定的高效液相色谱分析方法。加入内标物左美丙嗪后，用10%高氯酸将0.2 mL血清脱蛋白。用1 M碳酸钾溶液中和酸性上清液，离心除去不溶性盐。将一等份上清液应用于具有紫外检测（260 nm）的HPLC。在聚合物涂覆的ODS柱上实现HPLC分离，该柱用含有0.05%三氟乙酸、0.05%七氟丁酸和0.1%三乙胺的乙腈/水（40：60，v/v）平衡。标准曲线在0.05 - 50.0 µg/mL浓度范围内呈线性，检测下限为0.05 µg/mL。在0.5 µg/mL和5.0 µg/mL浓度下评价了方法的准确度和精密度。变异系数范围为4.0 ~ 4.5%。对一例疑似静脉注射葡萄糖酸氯己定后死亡的患者血清中氯己定浓度进行了测定。
A simple and reliable high-performance liquid chromatographic (HPLC) method for analyzing chlorhexidine in human serum was developed. After the addition of an internal standard, levomepromazine, 0.2 mL serum was deproteinized with 10% perchloric acid. The acidic supernatant was neutralized with 1M potassium carbonate solution, and the insoluble salt was removed by centrifugation. An aliquot of the supernatant was applied to HPLC with UV detection (260 nm). HPLC separation was achieved on a polymer-coated ODS column equilibrated with acetonitrile/water containing 0.05% trifluoroacetic acid, 0.05% heptafluorobutyric acid, and 0.1% triethylamine (40:60, v/v). The calibration curve was linear in the concentration range from 0.05 to 50.0 µg/mL, and the lower limit of detection was 0.05 µg/mL. The accuracy and precision of the method were evaluated at concentrations of 0.5 µg/mL and 5.0 µg/mL. The coefficients of variation ranged from 4.0 to 4.5%. The concentration of chlorhexidine in the serum of a patient who died after a suspected intravenous injection of chlorhexidine gluconate was determined.